Nucleotide sequence of the lipoamide dehydrogenase gene of Escherichia coli K12.
Nucleotide sequence of the lipoamide dehydrogenase gene of Escherichia coli K12.
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大肠杆菌K12的硫辛酸酰胺脱氢酶基因的核苷酸序列。
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发表时间：
1983
期刊：
影响因子：
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通讯作者：
J. Guest
中科院分区：
文献类型：
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作者：
P. Stephens;H. Lewis;M. Darlison;J. Guest
已通过双脱氧链终止法测定了 1980 个碱基对 DNA 片段的核苷酸序列，该 DNA 片段包含编码大肠杆菌 K12 丙酮酸脱氢酶复合物的硫辛酸酰胺脱氢酶组分 (E3) 的 lpd 基因。 lpd结构基因包含1419个碱基对（473个密码子，不包括起始AUG密码子）。它前面有一个良好的启动子和一个优秀的核糖体结合位点，它以一个典型的不依赖于 rho 的终止子序列结束。结果证实，lpd 基因是与编码丙酮酸脱氢酶复合物的 E1 和 E2 成分的 ace 操纵子相关的独立基因，但不是其一部分。 lpd基因相对于DNA相应区域的限制性图谱的位置和转录极性与之前的遗传和感染后标记研究完全一致。从核苷酸序列预测的组成、Mr（50554或51274，如果包括FAD辅因子）、氨基末端序列和羧基末端序列与先前关于纯化酶的研究非常一致。该酶还与猪心酶的肽和其他序列已确定的吡啶核苷酸二硫化物氧化还原酶（人红细胞谷胱甘肽还原酶和质粒编码的汞还原酶）表现出显着程度的序列同源性。
The nucleotide sequence of a 1980-base-pair segment of DNA, containing the lpd gene encoding the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase complex of Escherichia coli K12, has been determined by the dideoxy chain-termination method. The lpd structural gene comprises 1419 base pairs (473 codons, excluding the initiating AUG codon). It is preceded by a good promoter and an excellent ribosome binding site and it ends with a typical rho-independent terminator sequence. The results confirm that the lpd gene is an independent gene linked to, but not part of, the ace operon that encodes the E1 and E2 components of the pyruvate dehydrogenase complex. The location and transcriptional polarity of the lpd gene relative to the restriction map of the corresponding region of DNA, are completely consistent with previous genetic and post-infection labelling studies. The composition, Mr (50554 or 51274 if the FAD cofactor is included), amino-terminal sequence and carboxy-terminal sequence predicted from the nucleotide sequence are in excellent agreement with previous studies on the purified enzyme. The enzyme also exhibits a remarkable degree of sequence homology with peptides of the pig heart enzyme and with other pyridine nucleotide disulphide oxidoreductases whose sequences have been defined: human erythrocyte glutathione reductase and plasmid-encoded mercuric reductase.