Oscillations of the snail genes in the presomitic mesoderm coordinate segmental patterning and morphogenesis in vertebrate somitogenesis
Oscillations of the snail genes in the presomitic mesoderm coordinate segmental patterning and morphogenesis in vertebrate somitogenesis
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DOI：
10.1016/j.devcel.2006.02.011
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发表时间：
2006-03-01
影响因子：
11.8
通讯作者：
Pourquié, O
中科院分区：
文献类型：
--
作者：
Dale, JK;Malapert, P;Pourquié, O
脊椎动物胚胎的体节是由近轴中胚层的体节分裂而成。在这个过程中的体节发生的时间和空间的严格控制是由分割时钟和FGF信号波阵面。在这里，我们报告的蜗牛1和蜗牛2在小鼠和鸡的前体中胚层（PSM），分别环状mRNA的表达。而蜗牛基因的振荡是独立的NOTCH信号，我们表明，他们需要WNT和FGF信号。在鸡胚中过度表达Snail2阻止PSM中的环状Lfng和Meso1表达并破坏体节形成。此外，错误表达Snail 2的细胞不能表达Paraxis，保持间充质，从而被抑制经历最终形成上皮体节的上皮化事件。因此，蜗牛基因定义了一类协调分割和PSM形态发生的循环基因。
The segmented body plan of vertebrate embryos arises through segmentation of the paraxial mesoderm to form somites. The tight temporal and spatial control underlying this process of somitogenesis is regulated by the segmentation clock and the FGF signaling wave-front. Here, we report the cyclic mRNA expression of Snail1 and Snail2 in the mouse and chick presomitic mesoderm (PSM), respectively. Whereas Snail genes' oscillations are independent of NOTCH signaling, we show that they require WNT and FGF signaling. Over-expressing Snail2 in the chick embryo prevents cyclic Lfng and Meso1 expression in the PSM and disrupts somite formation. Moreover, cells misexpressing Snail2 fail to express Paraxis, remain mesenchymal, and are thereby inhibited from undergoing the epithelialization event that culminates in the formation of the epithelial somite. Thus, Snail genes define a class of cyclic genes that coordinate segmentation and PSM morphogenesis.