Anterior-posterior guidance of commissural Axons by Wnt-frizzled signaling
Anterior-posterior guidance of commissural Axons by Wnt-frizzled signaling
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DOI：
10.1126/science.1089610
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发表时间：
2003-12-12
期刊：
影响因子：
56.9
通讯作者：
Zou, YM
中科院分区：
文献类型：
--
作者：
Lyuksyutova, AI;Lu, CC;Zou, YM
哺乳动物背侧脊髓中的连合神经元将轴突腹向底板，在那里它们穿过中线，向前转向大脑；脊髓内的一种化学吸引剂(S)梯度控制着这种转向。在啮齿动物中，几种Wnt蛋白刺激中线交叉(后交叉)后连合轴突的延伸。我们发现WNT4信使RNA在底板中以从前到后的递减梯度表达，并且WNT4蛋白的直接来源吸引了交叉连合后的轴突。缺乏Wnt受体Frizzled3的小鼠的连合轴突在中线交叉后显示出前后导向缺陷。因此，WNT-Frizzled型信号引导连合轴突沿着脊髓的前后轴线。
Commissural neurons in the mammalian dorsal spinal cord send axons ventrally toward the floor plate, where they cross the midline and turn anteriorly toward the brain; a gradient of chemoattractant(s) inside the spinal cord controls this turning. In rodents, several Wnt proteins stimulate the extension of commissural axons after midline crossing (postcrossing). We found that Wnt4 messenger RNA is expressed in a decreasing anterior-to-posterior gradient in the floor plate, and that a directed source of Wnt4 protein attracted postcrossing commissural axons. Commissural axons in mice lacking the Wnt receptor Frizzled3 displayed anterior-posterior guidance defects after midline crossing. Thus, Wnt-Frizzled signaling guides commissural axons along the anterior-posterior axis of the spinal cord.