A taxon-specific oligonucleotide primer set for PCR-based detection of soil ciliate
A taxon-specific oligonucleotide primer set for PCR-based detection of soil ciliate
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DOI：
10.1264/jsme2.22.78
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发表时间：
2007-01-01
影响因子：
2.2
通讯作者：
Shimano, Satoshi
中科院分区：
文献类型：
--
作者：
Puitika, Tunjung;Kasahara, Yasuhiro;Shimano, Satoshi
设计了一对PCR引物，用于扩增纤毛门（纤毛虫）的18S rRNA基因。这对扩增的DNA来自5种生物，代表了纤毛虫的主要分类群，但没有来自13种非纤毛虫生物。为了测试引物从环境样品中扩增纤毛虫rRNA基因的能力，从农业土壤样品中构建克隆文库并进行分析。所有克隆的rRNA基因序列均归属于纤毛门。该PCR引物能够从土壤中扩增出纤毛虫rRNA基因，为纤毛虫的检测和鉴定提供了一条新的途径。
A PCR primer set was designed to amplify the 18S rRNA gene from the phylum Ciliophora (ciliates). The pair amplified DNA from 5 organisms representing the major taxonomic groups of ciliates but not from 13 nonciliate organisms. To test the ability of the primers to amplify ciliate rRNA genes from environmental samples, a clone library was constructed from an agricultural soil sample and analyzed. The rRNA gene sequences of all clones were affiliated with the Ciliophora. This newly developed PCR primer set is able to amplify ciliate rRNA genes from soil, and may be useful for the detection and identification of ciliates.