TEMPERATURE THRESHOLD LEVELS FOR HEAT-INDUCED BONE TISSUE-INJURY - A VITAL-MICROSCOPIC STUDY IN THE RABBIT
TEMPERATURE THRESHOLD LEVELS FOR HEAT-INDUCED BONE TISSUE-INJURY - A VITAL-MICROSCOPIC STUDY IN THE RABBIT
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DOI：
10.1016/0022-3913(83)90174-9
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发表时间：
1983-01-01
影响因子：
4.6
通讯作者：
ALBREKTSSON, T
中科院分区：
文献类型：
--
作者：
ERIKSSON, AR;ALBREKTSSON, T
如果骨细胞被手术准备过程中产生的摩擦热严重损伤，则骨手术后的愈合可能被延迟甚至被阻止。然而，对引起可逆或不可逆骨损伤的临界温度知之甚少。这个临界温度通常被认为是大约56 ℃，因为碱性磷酸酶在这个温度水平下变性。5-s研究表明，即使没有超过碱性磷酸酶的变性点，骨对低于56 ℃的温度的反应也可能导致骨坏死。Rouiller和Majno描述了将兔跖骨或桡骨加热至55 ℃ 3分钟后的硬组织损伤。在一个兔子实验模型中，Lundskog 'O发现了一些组织化学证据，表明在植入的骨组织附近，骨组织被加热到50摄氏度30秒。在这些研究和其他研究中，间接方法（组织学和组织化学）已用于评价硬组织活力。其他研究表明，传统的组织学评价往往低估了组织死亡的真实范围。“-13缺乏对骨组织临界温度的认识的一个原因是定义骨坏死的方法上的缺陷。此外，重要的问题不是骨是否会死亡，而是它是否会作为分化的组织存活下来。对加热后硬组织的短期和长期反应的可靠研究需要一种直接的方法，该方法允许在不同的随访时间对同一骨室进行重复原位研究。为满足这些要求，构建了用于加热骨组织活体显微镜检查的热室。14在这项研究中，热室被应用于一项旨在分析
H ealing following bone surgery may be delayed or even prevented if the bone cells are severely injured by the frictional heat generated during the surgical preparation. lT4 However, little is known about the critical temperature that causes a reversible or irreversible bone injury. This critical temperature is popularly believed to be around 56” C because alkaline phosphatase is denatured at this temperature level. 5-s Studies in which bone reactions to temperatures lower than 56” C have been investigated indicate that bone necrosis may result even if the denaturation point of alkaline phosphatase is not exceeded. Rouiller and Majno’described hard tissue injury after heating the rabbit metatarsal or radial bone to 55” C for 3 minutes. In a rabbit experimental model, Lundskog’O found some histochemical evidence of bone death adjacent to an implanted scald that was heated to 50” C for 30 seconds. In both these investigations and others, indirect methods (histology and histochemistry) have been used for evaluating hard tissue viability. Other studies have shown that conventional histologic evaluations tend to underestimate the true extension of tissue death.“-13 One reason for the lack of knowledge of the critical temperature of bone tissue is the methodologic shortcoming of defining bone necrosis. Furthermore, the important question is not whether the bone will die, but whether it will survive as a differentiated tissue.Reliable studies on the short-and long-term hard tissue reactions after heating demand a direct method that allows for repeated in situ studies of the same bone compartment at varying times of follow-up. The thermal chamber for intravital microscopy of heated bone tissue was constructed to meet these demands. 14 In this investigation the thermal chamber was applied in an investigation that sought to analyze the