(-)-Epicatechin maintains endurance training adaptation in mice after 14 days of detraining
(-)-Epicatechin maintains endurance training adaptation in mice after 14 days of detraining
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DOI：
10.1096/fj.11-196154
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发表时间：
2012-04-01
期刊：
影响因子：
4.8
通讯作者：
Malek, Moh H.
中科院分区：
文献类型：
--
作者：
Huettemann, Maik;Lee, Icksoo;Malek, Moh H.
本研究的目的是确定（-）-表儿茶素（主要存在于可可中）是否可以减弱小鼠后肢肌肉的停训效应。将32只雄性小鼠随机分为4组：对照组、训练组、停训14 d+溶媒组（DT-14-W）、停训14 d+（-）-表儿茶素组（DT-14-（-）-Epi）。DT-14-（-）-Epi组给予（-）-表儿茶素（1.0 mg/kg × 2X/d），DT-14-W组给予水。后3组均进行5周的耐力训练，5次/周。收获后肢肌肉，进行蛋白质印迹和酶分析。与对照组相比，训练显著增加了毛细血管与纤维的比率（约78.8%），细胞色素c氧化酶（约35%）和活性（约144%）。对于DT-14-W组，这些适应恢复到对照水平，而DT-14-（-）-Epi组能够维持高于对照水平的毛细血管与纤维比率（约44%）、CcO蛋白表达（约45%）和活性（约108%）。此外，毛细作用的增加与血小板反应蛋白-1（一种抗血管生成调节因子）的蛋白表达减少有关。此外，训练组和DT-14-（-）-Epi组之间的耐力没有显著差异。我们的数据表明，（-）-表儿茶素可能是一种合适的化合物，即使在运动方案停止时，也能维持运动诱导的毛细血管和线粒体能力的改善。Huttemann，M.，李岛，加-地Malek，M. H.（-）-表儿茶素维持停训14 d后小鼠的耐力训练适应。FASEB J.26，1413-1422（2012）。www.fasebj.org
The purpose of this study was to determine whether (-)-epicatechin (mainly found in cocoa) could attenuate detraining effects in the hindlimb muscles of mice. Thirty-two male mice were randomized into 4 groups: control, trained, trained with 14 d of detraining and vehicle (DT-14-W), and trained with 14 d of detraining and (-)-epicatechin [DT-14-(-)-Epi]. DT-14-(-)-Epi received (-)-epicatechin (1.0 mg/kg2X/d), whereas water was given to the DT-14-W group. The latter 3 groups performed 5 wk of endurance training 5X/wk. Hindlimb muscles were harvested, and Western blots, as well as enzyme analyses, were performed. Training significantly increased capillary-to-fiber ratio (approximate to 78.8%), cytochrome-c oxidase (approximate to 35%), and activity (approximate to 144%) compared to controls. These adaptations returned to control levels for the DT-14-W group, whereas the DT-14-(-)-Epi group was able to maintain capillary-to-fiber ratio (approximate to 44%), CcO protein expression (approximate to 45%), and activity (approximate to 108%) above control levels. In addition, the increase in capillarity was related to decreased protein expression of thrombospondin-1, an antiangiogenic regulator. Furthermore, there were no significant differences in endurance capacity between the trained and DT-14-(-)-Epi groups. Our data suggest that (-)-epicatechin may be a suitable compound to maintain exercise-induced improved capillarity and mitochondrial capacity, even when exercise regimens are discontinued.-Huttemann, M., Lee, I., Malek, M. H. (-)-Epicatechin maintains endurance training adaptation in mice after 14 d of detraining. FASEB J. 26, 1413-1422 (2012). www.fasebj.org