From dendrites to networks: Optically probing the living brain slice and using principal component analysis to characterize neuronal morphology
From dendrites to networks: Optically probing the living brain slice and using principal component analysis to characterize neuronal morphology
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DOI：
10.1007/0-387-28942-9_15
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发表时间：
2006-01-01
期刊：
影响因子：
--
通讯作者：
Yuste, Rafael
中科院分区：
文献类型：
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作者：
Goldberg, Jesse H.;Hamzei-Sichani, Farid;Yuste, Rafael
最近，活体脑片制备的光学成像技术的进步使得可以在多个水平上检查神经元电路，从树突上的单个突触接触到网络中的整个神经元群体。在这一章中，我们描述了三种技术，这些技术结合在一起，能够有效地解剖多个空间尺度上的神经元电路。我们描述了(1)结合全细胞记录、双光子钙成像和电子显微镜重建来检查突触刺激过程中单个突触和树突的功能的方法，(2)同时对大脑切片中的数百个神经元进行成像以检查活的神经元网络中活动的时空动力学的方法，以及(3)根据不同神经元亚类的多参数结构多样性而越来越有必要对神经元形态进行公正的、定量的分析。
Recently, advances in optical imaging of the living brain slice preparation have permitted neuronal circuitry to be examined at multiple levels, ranging from individual synaptic contacts on dendrites to whole populations of neurons in a network. In this chapter, we describe three techniques that, together, enable a powerful dissection of neuronal circuits across multiple space scales. We describe methods for (1) combining whole-cell recording with two-photon calcium imaging and electron microscopic reconstruction to examine the functions of individual synapses and dendrites during synaptic stimulation, (2) imaging hundreds of neurons in the brain slice simultaneously to examine the spatiotemporal dynamics of activity in living neuronal networks, and (3) performing an unbiased, quantitative analysis of neuronal morphology that is increasingly necessary in light of the multiparametric structural diversity of distinct neuronal subclasses.