Video-rate confocal scanning laser microscope for imaging human tissues in vivo
Video-rate confocal scanning laser microscope for imaging human tissues in vivo
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DOI：
10.1364/ao.38.002105
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发表时间：
1999-04-01
期刊：
影响因子：
1.9
通讯作者：
Webb, RH
中科院分区：
文献类型：
--
作者：
Rajadhyaksha, M;Anderson, RR;Webb, RH
我们建立了一台视频率共聚焦扫描激光显微镜，用于活体人体皮肤和口腔粘膜的反射成像。确定了最佳分辨率和对比度的设计和成像参数。制作了机械皮肤夹具和口腔组织夹，以稳定物镜与组织的接触，从而最大限度地减少相对于显微镜的组织运动。在1064 nm波长处，共焦成像的最大深度可达350微米的人体皮肤和450微米的口腔粘膜，横向分辨率为0.5-1微米，轴向分辨率(断面厚度)为3-5微米。这种分辨率可与传统的切除活检(组织学)显微镜相媲美。观察正常和异常的组织形态和动态过程。(C)1999年美国光学学会。
We have built a video-rate confocal scanning laser microscope for reflectance imaging of human skin and oral mucosa in vivo. Design and imaging parameters were determined for optimum resolution and contrast. Mechanical skin-holding fixtures and oral tissue clamps were made for stable objective lens-to-tissue contact such that gross tissue motion relative to the microscope was minimized. Confocal imaging was possible to maximum depths of 350 mu m in human skin and 450 mu m in oral mucosa, with measured lateral resolution of 0.5-1 mu m and axial resolution (section thickness) of 3-5 mu m at the 1064-nm wavelength. This resolution is comparable with that of conventional microscopy of excised biopsies (histology). Normal and abnormal tissue morphology and dynamic processes were observed. (C) 1999 Optical Society of America.