EXTRACELLULAR-MATRIX PROTEINS AND THEIR RECEPTORS IN THE NORMAL, HYPERPLASTIC AND NEOPLASTIC BREAST
EXTRACELLULAR-MATRIX PROTEINS AND THEIR RECEPTORS IN THE NORMAL, HYPERPLASTIC AND NEOPLASTIC BREAST
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DOI：
10.1016/0922-3371(90)90057-4
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发表时间：
1990-12-01
期刊：
影响因子：
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通讯作者：
VIRTANEN, I
中科院分区：
文献类型：
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作者：
GOULD, VE;KOUKOULIS, GK;VIRTANEN, I
我们通过免疫组织化学研究了生腱蛋白 (Ten)、细胞纤连蛋白 (cFn)、层粘连蛋白和某些相关细胞外基质蛋白受体在正常人类女性乳房、纤维囊性疾病 (FCD) 变体、良性肿瘤以及导管癌和小叶癌中的分布。 使用含有cFn的Ten域外A（EDAcFn）、层粘连蛋白A链和B链以及整合素的β-1（β-1）和不同α亚基的单克隆抗体（mAb）。在原位导管癌和小叶癌中，层粘连蛋白染色具有局灶性间隙，Ten-免疫反应性显示导管周围或腺泡周围条带，cFn显示广泛而强烈的导管周围染色；在非肿瘤性肌上皮细胞的基底细胞质中观察到β-1和α-6的强烈反应，而少数肿瘤细胞呈弱染色。 在浸润性导管癌和小叶癌（IDC、ILC）中，肿瘤簇周围的层粘连蛋白反应性较弱、不均匀或不存在，而 Ten 和 cFn 的基质染色广泛且强烈。 在大多数 IDC 中，中度 β-1 和 α-6 染色涉及不同的亚群；一种粘液癌染色强烈且弥漫。 在 ILC 中 20-40% 的细胞中，β-1 和 α-6 位于精致、分支的细胞质突起中。 mAbs对其他α-整合素亚基的间接免疫荧光研究表明，在各种乳腺癌中，肿瘤细胞中仅表达α-3，而血管中含有α-1整合素。与正常乳腺、FCD和良性肿瘤相比，乳腺癌中Ten和cFn的反应性增加，而层粘连蛋白减弱、减少或缺失；然而，Ten 不能被视为癌症标志物，因为它可以在良性肿瘤、FCD 甚至正常乳房中检测到。 在所有乳腺癌中，β-1 和 α-6 整合素亚基的反应性均降低；然而，小叶癌与导管癌中相对强烈且明显的局部反应可能很好地反映了这两种主要乳腺癌表型之间的生物学和临床差异。
We studied by immunohistochemistry, the distribution of tenascin (Ten), cellular fibronectin (cFn), laminin and certain pertinent extracellular matrix protein receptors in normal human female breast, variants of fibrocystic disease (FCD), benign tumors, and ductal and lobular carcinomas. Monoclonal antibodies (mAb) to Ten, extradomain A containing cFn (EDAcFn), A and B chains of laminin, and beta-1 (beta-1) and different alpha subunits of integrins were used.In in-situ ductal and lobular carcinomas, laminin staining has focal gaps, Ten-immunoreactivity displayed periductal or periacinar bands, and cFn showed broad and intense periductal staining; strong reactions for beta-1 and alpha-6 were noted in the basal cytoplasm of non-neoplastic myoepithelial cells while few tumor cells stained weakly. In infiltrating ductal and lobular carcinomas (IDC, ILC), laminin reactivity was weak, uneven or absent around neoplastic clusters whereas stromal staining for Ten and cFn was extensive and strong. In most IDC, moderate beta-1 and alpha-6 staining involved variable subpopulations; one mucinous carcinoma stained strongly and diffusely. In 20-40% of cells in ILC, beta-1 and alpha-6 were localized in delicate, ramified cytoplasmic processes. Indirect immunofluorescence studies with mAbs to other alpha-integrin subunits suggest that in various breast carcinomas only alpha-3 is expressed in tumor cells and that the vessels contained alpha-1 integrin.As compared with the normal breast, FCD and benign tumors, reactivity for Ten and cFn is increased in breast carcinomas while laminin is attenuated and decreased or absent; yet Ten cannot be regarded as a carcinoma marker since it can be detected in benign tumors, FCD, and even in the normal breast. Reactivity for beta-1 and alpha-6 integrin subunits is decreased in all breast carcinomas; however, the comparatively strong and distinctly localized reactions noted in lobular vs ductal carcinomas may well reflect biological and clinical differences between these two main breast carcinoma phenotypes.