Uniglomerular projection neurons participate in early development of olfactory glomeruli in the moth Manduca sexta.
Uniglomerular projection neurons participate in early development of olfactory glomeruli in the moth Manduca sexta.
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单肾小球投射神经元参与天蛾 Manduca sexta 嗅觉肾小球的早期发育。
DOI：
10.1002/cne.903500102
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Tolbert,LP
中科院分区：
文献类型：
--
作者：
Malun,D;Oland,LA;Tolbert,LP
触角（嗅觉）叶的肾小球组织是由来自触角的感觉轴突的到达启动的。轴突末端束合并成神经纤维球状结，称为原肾小球。先前的研究表明，原肾小球形成了成熟肾小球阵列的模板，但并未排除投射神经元在建立模板中的早期作用。我们用共焦激光扫描显微镜检查了肾小球构建阶段单肾小球投射神经元的形态发育。用亲脂性染料 DiI 对投射神经元组进行染色，以评估整个群体的发育情况；单个神经元的细胞内充满了荧光黄，以详细检查形状的发育。在一些制剂中，还使用不同的荧光染料标记感觉轴突和神经胶质细胞，以揭示投射神经元树突和感觉轴突或神经胶质细胞之间可能的相互作用。原肾小球以波的形式形成，从触角神经的入口点开始，穿过叶到相反的极点。接下来是第二波，其中投射神经元变得簇状并支配新形成的肾小球，有时延伸到原肾小球周围的神经胶质边界。在被剥夺感觉轴突的动物中，一些投射神经元仍然形成簇状树突树，并且在神经毡的一个区域形成肾小球样结构。原肾小球中早期存在投射神经元突起以及在未传入叶中形成至少一个肾小球样结构表明单肾小球投射神经元在嗅觉肾小球的构建中发挥着积极作用。 © 1994 Wiley-Liss, Inc.
Glomerular organization of the antennal (olfactory) lobe is initiated by the arrival of sensory axons from the antenna. Bundles of axon terminals coalesce into spheroidal knots of neuropil called protoglomeruli. Previous studies have suggested that the protoglomeruli form a template for the mature glomerular array, but an early role for projection neurons in establishing the template has not been excluded. We examined with the confocal laser scanning microscope the morphological development of the uniglomerular projection neurons during the stages in which glomeruli are constructed. Groups of projection neurons were stained with the lipophilic dye DiI to assess the development of the population as a whole; individual neurons were filled intracellularly with Lucifer Yellow to examine in detail the development of shape. In some preparations, sensory axons and glial cells also were labeled by using different fluorescent dyes to reveal possible interactions between projection neuron dendrites and sensory axons or glial cells.Protoglomeruli form in a wave beginning at the entry point of the antennal nerve and proceeding across the lobe to the opposite pole. A second wave follows in which projection neurons become tufted and innervate the newly formed glomeruli, sometimes extending into the glial border surrounding the protoglomeruli. In animals deprived of sensory axons, some projection neurons still form tufted dendritic trees and, in one region of the neuropil, a glomerulus‐like structure.The early presence of projection neuron processes in the protoglomeruli and the formation of at least one glomerulus‐like structure in unafferented lobes suggest that uniglomerular projection neurons play an active role in the construction of olfactory glomeruli. © 1994 Wiley‐Liss, Inc.