OPTIMIZATION OF 2,3-BUTANEDIOL PRODUCTION BY KLEBSIELLA-OXYTOCA THROUGH OXYGEN-TRANSFER RATE CONTROL
OPTIMIZATION OF 2,3-BUTANEDIOL PRODUCTION BY KLEBSIELLA-OXYTOCA THROUGH OXYGEN-TRANSFER RATE CONTROL
复制标题
DOI：
10.1002/bit.260421102
复制
发表时间：
1993-12-01
影响因子：
3.8
通讯作者：
TSAO, GT
中科院分区：
文献类型：
--
作者：
BERONIO, PB;TSAO, GT
产2,3-丁二醇的克雷伯氏菌受氧气限制程度的影响。在批量培养研究中，观察到两个生长阶段：能量耦合生长，在此期间细胞生长和氧气供应耦合；当氧气限制程度达到临界值时，会出现能量不耦合生长。最佳2,3-丁二醇产量发生在能量耦合生长阶段。本文介绍了一种在能量耦合生长中使间歇培养维持在一定限氧水平的控制系统。制度已经设计好了。控制包括对氧传递系数的反馈控制，通过不断增加原料气中氧的分压来实现，这反过来又不断增加氧传递速率。控制导致了平衡的生长状态，抑制了乙醇的形成，2,3-丁二醇的产量增加了18%。1993 John Wiley & Sons, Inc.；
Production of 2,3-butanediol by Klebsiella oxytoca is influenced by the degree of oxygen limitation. During batch culture studies, two phases of growth are observed: energy-coupled growth, during which cell growth and oxygen supply are coupled; and, energy-uncoupled growth, which arises when the degree of oxygen limitation reaches a critical value. Optimal 2,3-butanediol productivity occurs during the energy-coupled growth phase. In this article, a control system which maintains the batch culture at a constant level of oxygen limitation in the energy-coupled growth.regime has been designed. Control, which involves feedback control on the oxygen transfer coefficient, is achieved by continually increasing the partial pressure of oxygen in the feed gas, which in turn continually increases the oxygen transfer rate. Control has resulted in a balanced state of growth, a repression of ethanol formation, and an increase in 2,3-butanediol productivity of 18%. (C) 1993 John Wiley & Sons, Inc.