Functional characterization of the pathogenesis-related protein family 10 gene, PgPR10-4, from Panax ginseng in response to environmental stresses
Functional characterization of the pathogenesis-related protein family 10 gene, PgPR10-4, from Panax ginseng in response to environmental stresses
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DOI：
10.1007/s11240-014-0505-5
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发表时间：
2014-09-01
影响因子：
3
通讯作者：
Yang, Deok-Chun
中科院分区：
文献类型：
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作者：
Kim, Yu-Jin;Jang, Moon-Gi;Yang, Deok-Chun
病原相关蛋白（Pathogenesis-related proteins，PRs）是高等植物抵抗生物和非生物胁迫，特别是病原菌侵染的重要防御系统。从14年生人参表达序列标签文库中分离到一个PR BetV 1的全长cDNA序列，由于与已分离的PgPR 10 s序列具有相似的同源性，我们将其命名为PgPR 10 -4。PgPR 10 -4基因编码一个477 bp的开放阅读框，其推导的蛋白含有158个氨基酸，与中华猕猴桃BetV 1变应原的同源性为53%。PgPR 10 -4基因在人参叶片中表达丰富，其转录产物在几种人参病原菌和非生物刺激（如强光和盐）下表现出差异上调模式。此外，PgPR 10 -4表达强烈响应于应激信号分子H2 O2和茉莉酸（JA），而每周响应于水杨酸和脱落酸。PgPR 10 -4在拟南芥中的过表达进一步证实了其在环境胁迫耐受性中的功能作用。对过表达PgPR 10 -4基因的T2转基因拟南芥植物的分析表明，对细菌和真菌感染的耐受性增强，但对盐胁迫没有耐受性。当我们用青色荧光蛋白融合蛋白标记时，发现PgPR 10 -4-定位于细胞质。从拟南芥转基因系中观察到的增强的抗真菌活性表明PgPR 10 -4可能通过JA信号通路参与防御相关机制。
Pathogenesis-related proteins (PRs) are known to function in higher plants as a protein-based defensive system against abiotic and biotic stress, particularly pathogen infections. A full-length cDNA sequence of PR BetV1 was isolated and characterized from a 14-year-old ginseng expressed sequence tags library and we named this as PgPR10-4, because of similar identities with previous isolated PgPR10s sequences. The PgPR10-4 gene encodes a 477 bp open reading frame and its deduced protein contains 158 amino acids with a 53 % identity with that of the Actinidia chinensis BetV1 allergen. The expression of PgPR10-4 gene was abundant in leaves and its transcripts showed differentially up-regulated patterns against several ginseng pathogens and abiotic stimuli such as high light and salinity. In addition, PgPR10-4 expression was strongly responsive towards the stress signaling molecules H2O2 and jasmonic acid (JA), while weekly responsive to salicylic acid and abscisic acid. A functional role of PgPR10-4 in environmental stress tolerance was further validated through its overexpression in Arabidopsis. An analysis of T2 transgenic Arabidopsis plants overexpressing the PgPR10-4 gene showed an enhanced tolerance to bacterial and fungal infection, but not to salt stress. When we tagged with cyan fluorescent protein fusion protein, the PgPR10-4-was found to localize to the cytoplasm. The enhanced antifungal activity observed from the Arabidopsis transgenic lines suggests the possible involvement of PgPR10-4 in a defense-related mechanism via the JA signaling pathway.