Holographic sensors for the detection of bacterial spores
Holographic sensors for the detection of bacterial spores
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DOI：
10.1016/j.bios.2007.06.006
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发表时间：
2007-11-30
影响因子：
12.6
通讯作者：
Lowe, C. R.
中科院分区：
文献类型：
--
作者：
Bhatta, D.;Christie, G.;Lowe, C. R.
描述了用于检测芽孢杆菌属物种孢子萌发和营养生长的全息传感器。反射全息图的制作使用的扩散方法的超细银溴化颗粒的分布到预先形成的聚合物膜，然后全息记录使用倍频Nd：YAG（532 nm）激光。全息重放波长或衍射强度的变化被用来描述一系列全息基质的溶胀行为或结构完整性，以响应细菌孢子萌发和营养代谢的各种胞外产物。二价金属离子敏感全息图含有甲基丙烯酸类似物的氨三乙酸（NTA）作为螯合单体，成功地用于监测钙离子释放过程中B。在添加样品的几分钟内，实时观察枯草芽孢杆菌孢子萌发;全息响应表现为萌发过程中衍射波长的16 nm蓝移。类似地，包含甲基丙烯酸（MAA）作为可电离单体的pH敏感全息图响应于与B萌发后的早期营养代谢相关的pH变化。巨大芽孢杆菌孢子;观察到在75 nm的全息重放波长中视觉上可感知的蓝移。酪蛋白和淀粉为基础的全息矩阵，通过适当的基板与丙烯酰胺的共聚反应制备，用于检测在后期阶段的B释放的外切酶。megaterium和B.枯草杆菌营养细胞生长;两种基质的全息响应可见为衍射强度的降低，这是由于酶裂解引起的渐进条纹破坏。使用上述全息传感器的范围对各种萌发和生长事件的组合监测提供了用于检测活细菌孢子的新颖的综合手段。(c)2007 Elsevier B. V.保留所有权利。
Holographic sensors for the detection of Bacillus species spore germination and vegetative growth are described. Reflection holograms were fabricated using a diffusion method for the distribution of ultra-fine silver bromide grains into pre-formed polymer films, followed by holographic recording using a frequency doubled Nd:YAG (532 nm) laser. Changes in holographic replay wavelength or diffraction intensity were used to characterise the swelling behaviour or structural integrity of a range of holographic matrices in response to various extracellular products of bacterial spore germination and vegetative metabolism. Divalent metal ion-sensitive holograms containing a methacrylated analogue of nitrilotriacetic acid (NTA) as the chelating monomer were successfully used to monitor Ca2+ ions released during B. subtilis spore germination in real-time, which was within minutes of sample addition; the holographic response manifested as a 16 nm blue-shift in diffraction wavelength over the progress of germination. Similarly, pH-sensitive holograms comprising methacrylic acid (MAA) as the ionisable monomer were responsive to changes in pH associated with early vegetative metabolism following germination of B. megaterium spores; a visually perceptible blue-shift in holographic replay wavelength of 75 nm was observed. Casein and starch-based holographic matrices, prepared by co-polymerisation of the appropriate substrate with acrylamide, were used to detect exo-enzymes released during later stages of B. megaterium and B. subtilis vegetative cell growth; holographic responses of both matrices were visible as a reduction in diffraction intensity due to progressive fringe disruption caused by enzymatic cleavage. The combined monitoring of various germination and growth events using the range of aforementioned holographic sensors provides a novel, comprehensive means for the detection of viable bacterial spores. (c) 2007 Elsevier B.V. All rights reserved.