CELLULAR LOCALIZATION OF HISTAMINE AND MONOAMINES IN GASTRIC MUCOSA OF MAN
CELLULAR LOCALIZATION OF HISTAMINE AND MONOAMINES IN GASTRIC MUCOSA OF MAN
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DOI：
10.1007/bf00309904
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发表时间：
1969-01-01
期刊：
影响因子：
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通讯作者：
OWMAN, C
中科院分区：
文献类型：
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作者：
HAKANSON, R;LILJA, B;OWMAN, C
对人胃的各个区域进行组织化学分析，以了解单胺和组胺的细胞定位，并通过放射分析来检测组氨酸脱羧酶活性。1.胃组胺仅位于肥大细胞中，均匀分布在粘膜和粘膜下层的整个厚度中。2.在任何区域中均未出现可检测到的组氨酸脱羧酶活性。3.5-羟色胺位于肠嗜铬细胞中，其数量随着时间的推移而增加体朝向胃窦。有证据表明，其中一些细胞受到肾上腺素能神经支配。4.将组织样本与l-DOPA一起孵育后，由于其绿色甲醛诱导的荧光（可能是由于细胞内形成的多巴胺），另一个细胞系统在显微镜下变得可见。它们的形态类似于肠嗜铬细胞，因此被称为“肠嗜铬样细胞”。这些细胞的区域分布与肠嗜铬细胞相似。5.肾上腺素能神经末梢从粘膜下层延伸到粘膜，在靠近上皮的固有层中走行。
Various regions of the human stomach were analyzed histochemically for the cellular localization of monoamines and histamine, and radiometrically for the detection of histidine decarboxylase activity.1.Gastric histamine is located exclusively in mast cells evenly distributed throughout the entire thickness of the mucosa and submucosa.2.No detectable histidine decarboxylase activity occurs in any of the regions.3.5-Hydroxytryptamine is located in enterochromaffin cells which increase in number from the corpus towards the antrum. There is evidence that some of these cells receive an adrenergic innervation.4.After incubation of tissue specimens withl-DOPA another system of cells becomes visible in the microscope owing to their green formaldehyde-induced fluorescence, probably due to dopamine formed intracellularly. Their morphology resembles that of the enterochromaffin cells and they have hence been termed “enterochromaffin-like”. The regional distribution of these cells is similar to that of the enterochromaffin cells.5.Adrenergic nerve terminals from the submucosa extend into the mucosa to run in the lamina propria close to the epithelium.