Differential acute effects of phenobarbital and 3-methylcholanthrene pretreatment on CCl 4 -induced hepatotoxicity in rats.
Differential acute effects of phenobarbital and 3-methylcholanthrene pretreatment on CCl 4 -induced hepatotoxicity in rats.
复制标题
苯巴比妥和 3-甲基胆蒽预处理对 CCl 4 诱导的大鼠肝毒性的不同急性影响。
DOI：
10.1016/0041-008x(72)90180-9
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发表时间：
1972
影响因子：
3.8
通讯作者：
N. Fausto
中科院分区：
文献类型：
--
作者：
K. A. Suarez;G. Carlson;G. Fuller;N. Fausto
苯巴比妥(PB)可显著增强吸入CCl4大鼠3小时后SGOT和SGPT活性的升高。然而，这些肝脏毒性参数在用3-甲基胆蒽(MC)预处理的大鼠中显著低于用该赋形剂和暴露在CCl4蒸气中的大鼠。PB可提高肝微体NADPH细胞色素c还原酶活性和CO结合色素含量，而MC对肝微体NADPH细胞色素c还原酶活性无明显影响。尽管CCl4暴露使PB组和MC组大鼠的CO结合色素含量分别降低了61%和39%，但微粒体NADPH细胞色素c还原酶活性仅分别降低了6%和20%。染毒后21h，PB组和MC组大鼠SGOT和SGPT值差异较大。此时的组织学证据显示，PB处理的动物广泛受损，MC处理的动物有节制作用。MC和PB对NADPH细胞色素c还原酶活性和CO结合色素含量的不同影响可能是MC对CCl4染毒大鼠的保护作用的机制之一。
Phenobarbital (PB) pretreatment significantly enhanced the rise in SGOT and SGPT activity immediately after a 3 hr exposure of rats to CCl4by inhalation. However, these parameters of hepatotoxicity were significantly lower in rats pretreated with 3-methylcholanthrene (MC) when compared to rats pretreated with the vehicle and exposed to CCl4vapor. Hepatic microsomal NADPH cytochrome c reductase activity and the amount of CO-binding pigment were elevated by PB pretreatment, but MC had no effect on hepatic microsomal NADPH cytochrome c reductase activity. Although CCl4exposure reduced CO-binding pigment content by 61% in PB pretreated and by 39% in MC-pretreated rats, microsomal NADPH cytochrome c reductase activity was reduced by only 6% and 20%, respectively. At 21 hr after exposure to CCl4, the difference in SGOT and SGPT values of the PB and MC pretreated rats was more divergent. Histologic evidence at this time revealed extensive damage in the PB pretreated animals and a sparing effect in the MC pretreated animals. The differential effects of MC and PB pretreatment on NADPH cytochrome c reductase activity and CO-binding pigment content may be responsible for the observed protective effect of MC in CCl4exposed rats.