The Drosophila phosphoinositide 3-kinase Dp110 promotes cell growth
The Drosophila phosphoinositide 3-kinase Dp110 promotes cell growth
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DOI：
10.1002/j.1460-2075.1996.tb01049.x
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发表时间：
1996-12-02
期刊：
影响因子：
11.4
通讯作者：
Waterfield, MD
中科院分区：
文献类型：
--
作者：
Leevers, SJ;Weinkove, D;Waterfield, MD
磷脂酰肌醇3-激酶（PI 3 Ks）已经在进化上多样的生物体范围中被鉴定，包括哺乳动物、果蝇、酵母、植物和网骨藻。它们被多种细胞外信号激活，并参与有丝分裂、分化和细胞存活，以及控制细胞骨架和细胞形状。在这里，我们描述了果蝇p110（Dp 110）的分子和功能分析。分离出全长Dp 110 cDNA，发现其编码的蛋白质全长与I类哺乳动物PI 3 Ks p110 α和p110 β同源。Dp 110在果蝇的翅盘和眼盘中过表达分别导致果蝇的翅和眼增大。与此相反，Dp 110的过度表达，包含一个突变预测导致催化活性的损失，导致更小的翅膀和眼睛。翅膀大小的改变是由于细胞大小和细胞数量的变化，而在眼睛中只检测到细胞大小的差异。这些数据意味着Dp 110在果蝇发育过程中的生长控制中的作用，并对其他生物体中I类PI 3 Ks的功能产生影响。
Phosphoinositide 3-kinases (PI3Ks) have been identified in an evolutionarily diverse range of organisms, including mammals, Drosophila, yeast, plants and Dictyostelium. They are activated by a multitude of extracellular signals and implicated in mitogenesis, differentiation and cell survival, as well as in the control of the cytoskeleton and cell shape. Here we describe the molecular and functional analysis of Drosophila p110 (Dp110). A full-length Dp110 cDNA was isolated and found to encode a protein homologous throughout its length to the class I mammalian PI3Ks p110 alpha and p110 beta. Overexpression of Dp110 in wing or eye imaginal discs resulted in flies with enlarged wings or eyes respectively. In contrast, overexpression of Dp110 containing a mutation predicted to result in the loss of catalytic activity resulted in smaller wings and eyes. The alterations in wing size result from changes in both cell size and cell number, whereas in the eye only differences in cell size were detected, These data imply a role for Dp110 in growth control during Drosophila development and have implications for the function of class I PI3Ks in other organisms.