Extraction kinetics, thermodynamics, rheological properties and anti-BVDV activity of the hot water assisted extraction of Glycyrrhiza polysaccharide
Extraction kinetics, thermodynamics, rheological properties and anti-BVDV activity of the hot water assisted extraction of Glycyrrhiza polysaccharide
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热水辅助提取甘草多糖的提取动力学、热力学、流变学性质及抗BVDV活性
DOI：
10.1039/d0fo00608d
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发表时间：
2020
期刊：
影响因子：
6.1
通讯作者：
Chen Jixiang
中科院分区：
文献类型：
--
作者：
Wang Yonggang;Wang Xinjian;Zhang Kang;Zhang Xuan;Li Shaowei;Li Yanlin;Fan Wenguang;Leng Feifan;Yang Mingjun;Chen Jixiang
研究了甘草多糖（GP）的提取动力学和热力学参数，并对其流变性能和抗病毒活性进行了评价。结果表明，萃取过程符合菲克第二扩散定律。在料液比为1:15 （g mL - 1）、提取时间为120 min、提取温度为80℃的条件下，得到最佳浓度为97.62 mg mL - 1。整个提取过程为自发的吸热过程。利用高效液相色谱（HPLC）、圆二色（CD）和傅里叶变换红外光谱（FT-IR）等手段证实GP是一种结构复杂的酸性糖蛋白。对其流变特性的研究表明，GP具有典型的非牛顿假塑性流体的特性，其粘度受温度、pH值和其他离子的存在显著影响。通过扫描电镜（SEM）和原子力显微镜（AFM）观察到具有不规则分子聚集的支状和软纤维结构。此外，GP通过调控MDBK细胞中IRF-1和IRF-3基因的相对表达水平，对牛病毒性腹泻病毒（BVDV）表现出良好的抑制活性。发现这种活性依赖于GP的物理化学和结构性质。这些发现表明，GP可以被认为是预防病毒性疾病的活性物质的天然来源。
The extraction kinetics and thermodynamic parameters of Glycyrrhiza polysaccharide (GP) were studied, and its rheological properties and antiviral activity were evaluated. The results showed that the extraction process could be fitted to Fick's second law of diffusion. The optimum concentration (97.62 mg mL−1) was obtained at a solid–liquid ratio of 1 : 15, (g mL−1), an extraction time of 120 min and an extraction temperature of 80 °C. The whole extraction process was spontaneous and endothermic. GP was shown to be an acid glycoprotein with a complex structure using high performance liquid chromatography (HPLC), circular dichroism (CD) and Fourier-transform infrared spectroscopy (FT-IR). A study of its rheological properties showed that GP has the characteristics of a typical non-Newtonian pseudoplastic fluid and that its viscosity could be significantly affected by temperature, pH and the presence of other ions. Branched and soft fiber structures with irregular molecular aggregation were observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Moreover, GP showed good inhibitory activity against bovine viral diarrhea virus (BVDV) via the regulation of the relative expression levels of the IRF-1 and IRF-3 genes in MDBK cells. This activity was found to be dependent on the physicochemical and structural properties of GP. These findings imply that GP can be considered as a natural source of active material for the prevention of viral disease.