The Hha protein facilitates incorporation of horizontally acquired DNA in enteric bacteria
The Hha protein facilitates incorporation of horizontally acquired DNA in enteric bacteria
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DOI：
10.1099/mic.0.062448-0
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发表时间：
2013-03-01
期刊：
影响因子：
2.8
通讯作者：
Juarez, Antonio
中科院分区：
文献类型：
--
作者：
Aznar, Sonia;Paytubi, Sonia;Juarez, Antonio
Hha样蛋白是肠杆菌科成员的进化特征。这些蛋白质模拟核相关蛋白H-NS的寡聚化结构域，并与后者蛋白质相互作用以调节基因表达。在这份报告中，我们提供的证据表明，已被证明为H-NS，HHA样蛋白发挥重要作用，促进收购水平转移的DNA在大肠杆菌和沙门氏菌。接合质粒如pHly 152或R27的引入导致E.大肠杆菌或沙门氏菌菌株缺乏Hha样蛋白。E.来自双hha ydgT突变体的大肠杆菌自发衍生物显示增加的生长速率和恢复的适应性过表达H-NS蛋白。除了加强H-NS/Hha调节异种DNA的作用外，所获得的结果还表明，肠杆菌科显示出在其他细菌中未发现的促进水平转移DNA掺入的调节特征。
Hha-like proteins are an evolutive trait of members of the family Enterobacteriaceae. These proteins mimic the oligomerization domain of the nucleoid-associated protein H-NS and interact with this latter protein to modulate gene expression. In this report, we provide evidence that, as has been shown for H-NS, Hha-like proteins play an essential role facilitating acquisition of horizontally transferred DNA in both Escherichia coli and Salmonella. Incorporation of conjugative plasmids such as pHly152 or R27 results in a fitness cost in E. coli or Salmonella strains that lack Hha-like proteins. E. coli spontaneous derivatives from double hha ydgT mutants that showed an increased growth rate and a restored fitness overexpressed the H-NS protein. In addition to reinforcing the role of H-NS/Hha-modulating xenogeneic DNA, the results obtained demonstrate that the Enterobacteriaceae display regulatory features not found in other bacteria that facilitate incorporation of horizontally transferred DNA.