Quantitative Analysis of B-Cell Subpopulations in Bone Marrow by Flow Cytometry.
Quantitative Analysis of B-Cell Subpopulations in Bone Marrow by Flow Cytometry.
复制标题
通过流式细胞术对骨髓中的 B 细胞亚群进行定量分析。
DOI：
10.1007/978-1-0716-2760-0_8
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Wang,Penghua
中科院分区：
文献类型：
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作者：
Geng,Tingting;Wang,Penghua
流式细胞术是一种快速检测和测量单个细胞或颗粒的物理和化学特性的技术。它是许多研究领域的强大工具，特别是免疫学，它允许同时分析组织中不同的免疫细胞群。在这里，我们描述的程序，以定量和/或纯化各种B馏分在小鼠骨髓流式细胞术使用其签名的表面标记。该方法可用于研究稳态或紧急造血过程中（如病毒感染）的B细胞发育。
Flow cytometry is a technology that rapidly detects and measures physical and chemical characteristics of single cells or particles. It is a powerful tool for many areas of research, in particular, immunology, which allows for simultaneous analysis of different immune cell populations in a tissue. Here we describe the procedures to quantify and/or purify various B fractions in mouse bone marrows by flow cytometry using their signature surface markers. This method is useful to study B-cell development during steady-state or emergency hematopoiesis such as viral infections.