HSP70 Gene Expression in the Zebrafish Retina After Optic Nerve Injury: A Comparative Study Under Heat Shock Stresses
HSP70 Gene Expression in the Zebrafish Retina After Optic Nerve Injury: A Comparative Study Under Heat Shock Stresses
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DOI：
10.1007/978-1-4614-0631-0_84
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发表时间：
2012-01-01
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影响因子：
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通讯作者：
Kato, Satoru
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文献类型：
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作者：
Fujikawa, Chieko;Nagashima, Mikiko;Kato, Satoru
与哺乳动物不同，鱼类视网膜神经节细胞（RGC）在视神经损伤（ONI）后可以再生轴突并恢复视觉功能，这是CNS修复的最佳模型。热休克蛋白70（HSP 70）在各种环境胁迫下对细胞的保护中起着重要作用。HSP 70是由磷酸化的热休克因子-1（pHSF-1）反式激活的。在本研究中，我们研究了斑马鱼（ZF）视网膜在ONI和热休克条件（37°C，30 min）后的HSP 70和pHSF-1表达。ONI后0.5 ~ 24 h，RGC内HSP 70持续升高，72 h后恢复到对照组水平。热休克后0.5 ~ 1h，各核层的HSP 70均短暂升高，3 h后恢复到对照组水平。热休克后ZF视网膜HSF-1 mRNA表达无明显变化，而ONI后0.5 ~ 24 h ZF视网膜HSF-1 mRNA表达明显增加。在热休克和ONI后均检测到pHSF-1。以上结果表明，ONI后HSP 70 mRNA表达的增加是由pHSF-1转位激活和HSF-1 mRNA在核内转录激活引起的，而热休克后HSP 70 mRNA表达的增加仅是由pHSF-1转位激活引起的。
Unlike mammals, fish retinal ganglion cells (RGCs) can regrow their axon and restore visual function after optic nerve injury (ONI), which is the best model for CNS repair. Heat shock protein 70 (HSP70) plays an important role for cell protection by various environmental stresses. HSP70 is transactivated by phosphorylated Heat shock factor-1 (pHSF-1). In this study, we investigate the expression of HSP70 and pHSF-1 after ONI and heat shock condition (at 37°C for 30 min) in zebrafish (ZF) retina. After ONI, HSP70 was increased for a long time in the RGCs during 0.5–24 h and then returned to the control level by 72 h. In contrast, HSP70 increased for a short time in all nuclear layers at 0.5–1 h, and then returned to control level by 3 h after heat shock. The level of HSF-1 mRNA was significantly increased in ZF retina 0.5–24 h after ONI, but not after heat shock. pHSF-1 was detected after both heat shock and ONI. These results indicate that the increased expression of HSP70 mRNA after ONI is caused by both activations of translocation of pHSF-1 and of transcription of HSF-1 mRNA in the nucleus, whereas the increased expression of HSP70 mRNA after heat shock is caused only by activation of pHSF-1 translocation into the nucleus.