THE AUTONOMOUS PARVOVIRUS MVM ENCODES 2 NON-STRUCTURAL PROTEINS IN ADDITION TO ITS CAPSID POLYPEPTIDES
THE AUTONOMOUS PARVOVIRUS MVM ENCODES 2 NON-STRUCTURAL PROTEINS IN ADDITION TO ITS CAPSID POLYPEPTIDES
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DOI：
10.1016/0042-6822(83)90172-1
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发表时间：
1983-01-01
期刊：
影响因子：
3.7
通讯作者：
TATTERSALL, P
中科院分区：
文献类型：
--
作者：
COTMORE, SF;STURZENBECKER, LJ;TATTERSALL, P
来自感染自主细小病毒MVM的小鼠L细胞的mRNA的体外翻译产生4种主要的病毒编码蛋白。这些蛋白质中的两种在抗原性上和通过肽图谱分析与病毒衣壳多肽VP-1和VP-2都无法区分。另外2种蛋白，命名为NS-1和NS-2，与衣壳多肽无关，但被来自感染不同自主细小病毒血清型的动物的血清识别。在体外制备的NS-1蛋白与VP-1（MW约为1000）共迁移。83，000），而NS-2多肽具有24，000的表观MW。NS-1多肽的转录物定位于位于MVM基因组中左侧转录单位的主要内含子中的开放阅读框的区块。
In vitro translation of mRNA from mouse L cells infected with the autonomous parvovirus MVM yields 4 major virally coded proteins. Two of these proteins are indistinguishable both antigenically and by peptide map analysis from the viral capsid polypeptides VP-1 and VP-2. The other 2 proteins, designated NS-1 and NS-2, are not related to the capsid polypeptides but are recognized by sera from animals infected with different autonomous parvovirus serotypes. The NS-1 protein made in vitro comigrates with VP-1 (MW .apprx. 83,000), while the NS-2 polypeptide has an apparent MW of 24,000. The transcript for the NS-1 polypeptide was mapped to a block of open reading frame located in the major intron of the left-hand transcription unit in the MVM genome.