A high performance microfluidic analyser for phosphate measurements in marine waters using the vanadomolybdate method
A high performance microfluidic analyser for phosphate measurements in marine waters using the vanadomolybdate method
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DOI：
10.1016/j.talanta.2013.05.004
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发表时间：
2013-11-15
期刊：
影响因子：
6.1
通讯作者：
Achterberg, Eric P.
中科院分区：
文献类型：
--
作者：
Legiret, Francois-Eric;Sieben, Vincent J.;Achterberg, Eric P.
报道了一种基于钒钼酸盐法测定海水中可溶性活性磷的高性能自动分析系统。该系统结合了由有色聚甲基丙烯酸甲酯（PMMA）制造的微流体芯片，定制的注射泵，嵌入式控制电子设备和板载校准标准。这种“芯片实验室”分析系统已成功部署，并与沿海（英格兰西南部）和公海沃茨（热带北大西洋）的参考分析方法进行了交叉比较。小型化系统的结果与参考台操作的磷酸盐自动分析仪进行了很好的比较，并且在分析结果中没有显示出显著差异（学生t检验，95%置信水平）。所使用的光学技术，包括着色的PMMA和抛光的流体通道，允许两个数量级的检测限（52 nM）相比，目前可用的便携式系统的基础上，这种方法的改进。该系统具有宽的线性动态范围0.1-60 μ M，和良好的精度（13.6%，0.4 μ M，n=4）。在0.7 μ M时对分析结果进行硅酸盐干扰校正，测量频率可配置为每小时最多20个样品的采样通量。这种便携式微量分析系统具有低试剂要求（每个样品340 μ L）和功耗（每个样品756 J），并允许海水中可溶性活性磷的精确高分辨率测量。(C)2013爱思唯尔有限公司版权所有。
We report a high performance autonomous analytical system based on the vanadomolybdate method for the determination of soluble reactive phosphorus in seawater. The system combines a microfluidic chip manufactured from tinted poly (methyl methaaylate) (PMMA), a custom made syringe pump, embedded control electronics and on-board calibration standards. This "lab-on-a-chip" analytical system was successfully deployed and cross-compared with reference analytical methods in coastal (south west England) and open ocean waters (tropical North Atlantic). The results of the miniaturized system compared well with a reference bench-operated phosphate auto-analyser and showed no significant differences in the analytical results (student's t-test at 95% confidence level). The optical technology used, comprising of tinted PMMA and polished fluidic channels, has allowed an improvement of two orders of magnitude of the limit of detection (52 nM) compared to currently available portable systems based on this method. The system has a wide linear dynamic range 0.1-60 mu M, and a good precision (13.6% at 0.4 mu M, n=4). The analytical results were corrected for silicate interferences at 0.7 mu M, and the measurement frequency was configurable with a sampling throughput of up to 20 samples per hour. This portable micro-analytical system has a low reagent requirement (340 mu L per sample) and power consumption (756J per sample), and has allowed accurate high resolution measurements of soluble reactive phosphorus in seawater. (C) 2013 Elsevier B.V. All rights reserved.