Green tea catechin (-)-epicatechin gallate induces tumour suppressor protein ATF3 via EGR-1 activation
Green tea catechin (-)-epicatechin gallate induces tumour suppressor protein ATF3 via EGR-1 activation
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DOI：
10.1016/j.ejca.2007.07.020
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发表时间：
2007-11-01
影响因子：
8.4
通讯作者：
Baek, Seung Joon
中科院分区：
文献类型：
--
作者：
Cho, Kyou-Nam;Sukhthankar, Mugdha;Baek, Seung Joon
表儿茶素没食子酸酯（ECG）是绿色茶中的第三大儿茶素成分，但它在某些方面显示出很强的生物活性，包括细胞凋亡、细胞生长抑制，以及各种细胞的膜转运系统。我们以前报道，ECG诱导激活转录因子3（ATF 3），这是参与在HCT-116细胞的促凋亡。本研究通过EMSA和共转染实验，探讨ECG诱导HCT-116细胞ATF 3基因表达的分子机制，发现Sp3参与了ATF 3基因的基础表达，而EGR-1在ECG诱导的HCT-116细胞ATF 3基因表达中起重要作用。这些结果表明，EGR-1，一种肿瘤抑制蛋白，可以证实ECG的ATF 3表达在人大肠癌细胞中的作用。我们还发现ECG的促氧化活性有助于ECG诱导的ATF 3表达。(c)2007爱思唯尔有限公司保留所有权利。
Epicatechin gallate (ECG) is the third major catechin component in green tea, but it shows strong biological activity in some aspects, including apoptosis, cell growth inhibition, and membrane transport system in various cells. We previously reported that ECG induces activating transcription factor 3 (ATF3), which is involved in pro-apoptosis in HCT-116 cells. In this report, we present a molecular mechanism by which ECG induces ATF3 expression at the transcriptional level.We found that Sp3 contributed to the basal expression of the ATF3 gene, whereas EGR-1 played an important role in ECG-induced ATF3 expression in HCT-116 cells, as assessed by EMSA and co-transfection experiments. These results suggested that EGR-1, a tumour suppressor protein, could substantiate ECG's role of ATF3 expression in human colorectal cancer cells. We also found that pro-oxidant activity of ECG contributed to ECG-induced ATF3 expression. (c) 2007 Elsevier Ltd. All rights reserved.