Sp1 plays an important role in regulating the transcription of ZNF313
Sp1 plays an important role in regulating the transcription of ZNF313
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DOI：
10.1042/cbi20100216
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发表时间：
2010-09
影响因子：
3.9
通讯作者：
Xiaolin Liao;Wenqian Deng;Yi-lu Lu;D. Tao;Yunqiang Liu;Si-zhong Zhang;Yongxin Ma
中科院分区：
文献类型：
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作者：
Xiaolin Liao;Wenqian Deng;Yi-lu Lu;D. Tao;Yunqiang Liu;Si-zhong Zhang;Yongxin Ma
ZNF 313基因在可育男性睾丸中的转录水平最高，可能与人类精子发生有关。构建ZNF 313启动子缺失突变质粒，转染HEK 293细胞。结果表明，nt-157至nt +8的片段具有基本的转录活性。通过定点突变试验和光神霉素A处理鉴定了功能性Sp1结合位点。在+233至-213处的447-bp显示出特征性的CpG岛，其与启动子区域重叠。我们的研究结果表明ZNF 313基因在转录水平受到调控，并且存在一种共同的调控机制。
The ZNF313 gene has the highest transcription level in fertile male testes and may be related to human spermatogenesis. The deletion‐mutated plasmids of ZNF313 promoter were constructed and transfected into HEK293 cells. The result showed that the fragment from nt −157 to +8 has a basal transcriptional activity. A functional Sp1 binding site was identified by site‐directed mutation test and mithramycin A treatment. A 447‐bp based at +233 to −213 exhibits a characteristic CpG island, which overlaps with the promoter region. Our work suggests that ZNF313 is controlled at the transcriptional level, and a common mechanism controlling the basal transcription of ZNF313 gene exists.