Acetyl-coenzyme A acyltransferase 2 attenuates the apoptotic effects of BNIP3 in two human cell lines
Acetyl-coenzyme A acyltransferase 2 attenuates the apoptotic effects of BNIP3 in two human cell lines
复制标题
乙酰辅酶 A 酰基转移酶 2 减弱 BNIP3 在两种人类细胞系中的凋亡作用
DOI：
10.1016/j.bbagen.2008.02.007
复制
发表时间：
2008-06-01
影响因子：
3
通讯作者：
Lu, Hong
中科院分区：
文献类型：
--
作者：
Cao, Wei;Liu, Nansong;Lu, Hong
BNIP 3是一种独特的促凋亡蛋白，属于Bcl-2家族的仅BH 3亚群，定位于线粒体膜上。尽管识别膜蛋白的结合伴侣存在固有的困难，但已经识别了BNIP 3的几种结合伴侣。本研究构建了一个改进的分裂泛素膜酵母双杂交系统，并用于鉴定乙酰辅酶A酰基转移酶2（ACAA 2）作为一个新的BNIP 3结合伴侣。BNIP 3和ACAA 2之间的相互作用通过pull-down和免疫共沉淀试验证实。ACAA 2也被发现与BNIP 3共定位在线粒体中。ACAA 2可抑制BNIP 3过表达诱导的人肝癌HepG 2细胞和骨肉瘤U-2 OS细胞凋亡。这些结果有力地表明ACAA 2是一个功能性的BNIP 3结合伴侣，并提供了脂肪酸代谢和细胞凋亡之间的可能联系。(c)2008 Elsevier B. V.保留所有权利。
BNIP3 is a unique pro-apoptotic protein which belongs to the BH3-only subset of the Bcl-2 family and localizes on mitochondrial membrane. Despite the inherent difficulty of identifying binding partners for membrane proteins, several binding partners for BNIP3 have been identified. In this study, a modified split-ubiquitin membrane yeast two-hybrid system was constructed and used to identify acetyl-Coenzyme A acyltransferase 2 (ACAA2) as a new BNIP3 binding partner. The interaction between BNIP3 and ACAA2 was confirmed by pull-down and co-immunoprecipitation assays. ACAA2 was also found to co-localize with BNIP3 in mitochondria. Furthermore, the apoptosis induced by over-expressed BNIP3 via transfection OF hypoxia treatment was abolished by ACAA2 in human hepatocellular carcinoma HepG2 cells and osteosarcoma U-2 OS cells. These results strongly suggest that ACAA2 be a functional BNIP3 binding partner and provide a possible linkage between fatty acid metabolism and apoptosis of cells. (c) 2008 Elsevier B.V. All rights reserved.