Principal Component and Cluster Analysis of Layer V Pyramidal Cells in Visual and Non-Visual Cortical Areas Projecting to the Primary Visual Cortex of the Mouse
Principal Component and Cluster Analysis of Layer V Pyramidal Cells in Visual and Non-Visual Cortical Areas Projecting to the Primary Visual Cortex of the Mouse
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DOI：
10.1093/cercor/bhs060
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发表时间：
2013-03-01
期刊：
影响因子：
3.7
通讯作者：
Boire, D.
中科院分区：
文献类型：
--
作者：
Laramee, M. E.;Rockland, K. S.;Boire, D.
位于第V层的锥体神经元产生的视觉和非视觉感觉区域之间的长距离皮质连接可被认为是反馈连接的一个亚群。本研究的目的是确定投射到视觉皮质(V1)的视觉和非视觉感觉皮质区域的V层锥体神经元是否构成一个同质的细胞群体。此外，我们询问树突分支是否与靶点、感觉通道、层级水平或来源皮质区域的偏侧性有关。对双侧大脑半球初级听觉(A1)和躯体感觉(S1)皮质以及次级视觉皮质的外侧(V2L)和内侧(V2M)部分的神经元进行逆行标记的V层锥体神经元树突的完整三维重建。对提取的形态参数进行主成分分析和聚类分析。主成分分析表明，神经元分布在一个连续的形态范围内，不形成离散的组。然而，集群分析定义了具有相似特征的神经元组。每个皮质区域包括属于几个簇的神经元。我们认为，单个皮质区域内的V层反馈连接由几种细胞类型组成。
The long-distance corticocortical connections between visual and nonvisual sensory areas that arise from pyramidal neurons located within layer V can be considered as a subpopulation of feedback connections. The purpose of the present study is to determine if layer V pyramidal neurons from visual and nonvisual sensory cortical areas that project onto the visual cortex (V1) constitute a homogeneous population of cells. Additionally, we ask whether dendritic arborization relates to the target, the sensory modality, the hierarchical level, or laterality of the source cortical area. Complete 3D reconstructions of dendritic arbors of retrogradely labeled layer V pyramidal neurons were performed for neurons of the primary auditory (A1) and somatosensory (S1) cortices and from the lateral (V2L) and medial (V2M) parts of the secondary visual cortices of both hemispheres. The morphological parameters extracted from these reconstructions were subjected to principal component analysis (PCA) and cluster analysis. The PCA showed that neurons are distributed within a continuous range of morphologies and do not form discrete groups. Nevertheless, the cluster analysis defines neuronal groups that share similar features. Each cortical area includes neurons belonging to several clusters. We suggest that layer V feedback connections within a single cortical area comprise several cell types.