Performance of the Chromogenic Medium CHROMagar Staph Aureus and the Staphychrom Coagulase Test in the Detection and Identification of Staphylococcus aureus in Clinical Specimens
Performance of the Chromogenic Medium CHROMagar Staph Aureus and the Staphychrom Coagulase Test in the Detection and Identification of Staphylococcus aureus in Clinical Specimens
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科玛嘉显色培养基金黄色葡萄球菌和葡萄球菌凝固酶试验在临床标本中金黄色葡萄球菌检测和鉴定中的性能
DOI：
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发表时间：
2001
影响因子：
9.4
通讯作者：
A. Freydiere
中科院分区：
文献类型：
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作者：
A. Carricajo;A. Treny;N. Fonsale;M. Bes;M. Reverdy;Y. Gille;G. Aubert;A. Freydiere
CHROMagar金黄色葡萄球菌（CSAM） （CHROMagar微生物学，巴黎，法国）是一种新的显色培养基，旨在通过金黄色葡萄球菌菌落的粉红色检测金黄色葡萄球菌。共775个标本在CHROMagar金黄色葡萄球菌和常规培养基上平行培养。在至少一种培养基上分离得到的267株金黄色葡萄球菌中，在CSAM培养基上分离到263株（敏感性98.5%），在常规培养基上分离到245株（敏感性91.8%）。根据CSAM培养基上菌落的粉红色推测金黄色葡萄球菌的特异性为97%（508例中的493例）。添加Staphychrom凝固酶检测后，特异性提高到100%；添加Pastorex Staph Plus凝集检测金黄色葡萄球菌表面成分时，特异性提高到98.8%。67株金黄色葡萄球菌的药敏试验，在粉红色CSAM菌落和生长在血琼脂上的菌落上平行进行，结果相似。因此，以CSAM为主要分离培养基，再进行葡萄凝固酶staphylo凝固试验，实现了对金黄色葡萄球菌的快速准确识别和鉴定。可直接对粉色CSAM菌落进行药敏试验（纸片扩散法或ATB STAPH系统）。
ABSTRACT CHROMagar Staph aureus (CSAM) (CHROMagar Microbiology, Paris, France) is a new chromogenic medium designed to enable detection of colonies of Staphylococcus aureus by their pink color. A total of 775 specimens were cultured in parallel on CHROMagar Staph aureus and conventional media. Among the 267 S. aureus strains recovered on at least one medium, 263 were isolated on CSAM medium (sensitivity, 98.5%), and 245 (sensitivity, 91.8%) were isolated on conventional media. The specificity of presumptive identification of S. aureus on the basis of pink colony color on CSAM medium was 97% (493 of 508). This specificity increased to 100% when coagulase detection with the Staphychrom coagulase test was added and to 98.8% when S. aureus surface components were detected by agglutination in the Pastorex Staph Plus test. Susceptibility testing of 67 S. aureus strains, performed in parallel on pink CSAM colonies and on colonies grown on blood agar, gave similar results. Thus, rapid and accurate recognition and identification of S. aureusisolates were achieved with CSAM as the primary isolation medium, followed by the staphylocoagulase Staphychrom test. Antimicrobial susceptibility testing (disk-diffusion method or ATB STAPH System) can be performed directly on pink CSAM colonies.