uDENN, DENN, and dDENN: Indissociable domains in Rab and MAP kinases signaling pathways
uDENN, DENN, and dDENN: Indissociable domains in Rab and MAP kinases signaling pathways
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DOI：
10.1006/bbrc.2001.5652
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发表时间：
2001-09-28
影响因子：
3.1
通讯作者：
Callebaut, I
中科院分区：
文献类型：
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作者：
Levivier, E;Goud, B;Callebaut, I
DENN 结构域存在于多种信号蛋白中，但其确切功能仍不清楚。一些包含 DENN 的蛋白质，例如大鼠 Rab3GEP（Rab3 GDP/GTP 交换蛋白）或小鼠 Rab6IP1（Rab6 相互作用蛋白 1）与 Rab 家族的 GTPases 相互作用。其他基因产物，例如人 MADD（含有死亡结构域的 MAP（丝裂原激活蛋白）激酶激活蛋白）和人 ST5（细胞分化抑制因子 5）基因产物，参与 MAPK（丝裂原激活蛋白激酶）信号通路的调节。通过结合基于配置文件和二维分析，我们在此表明 DENN 域比迄今为止在域数据库中描述的要大得多，两侧总是被更不同的域包围，我们将其称为 uDENN 和 dDENN。然而，它们共享保守氨基酸，这些氨基酸可能在 DENN 功能中发挥关键作用。 (C) 2001 年学术出版社。
DENN domains are found in a variety of signaling proteins but their exact function remains undefined. Some of the DENN-containing proteins, such as rat Rab3GEP (Rab3 GDP/GTP exchange protein) or mouse Rab6IP1 (Rab6 interacting protein 1) interact with GTPases of the Rab family. Others, such as human MADD (MAP (Mitogen-activated protein) kinase activating protein containing death domain) and human ST5 (Suppressor of turnoreginicity 5) gene products are involved in regulation of MAPKs (Mitogen-activated protein kinases) signaling pathways. Using a combination of profile-based and bidimensional analyses, we show here that DENN domains are much larger than described to date in domain databases, always encircled on both sides by more divergent domains, that we called uDENN and dDENN. These however share conserved amino acids which could play a key role in the DENN functions. (C) 2001 Academic Press.