INDIVIDUAL NEURONS DISSOCIATED FROM RAT SUPRACHIASMATIC NUCLEUS EXPRESS INDEPENDENTLY PHASED CIRCADIAN FIRING RHYTHMS
INDIVIDUAL NEURONS DISSOCIATED FROM RAT SUPRACHIASMATIC NUCLEUS EXPRESS INDEPENDENTLY PHASED CIRCADIAN FIRING RHYTHMS
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DOI：
10.1016/0896-6273(95)90214-7
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发表时间：
1995-04-01
期刊：
影响因子：
16.2
通讯作者：
REPPERT, SM
中科院分区：
文献类型：
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作者：
WELSH, DK;LOGOTHETIS, DE;REPPERT, SM
在哺乳动物下丘脑中，视交叉上核（SCN）包含一个生物钟，用于定时各种神经元、内分泌和行为节律。通过在固定微电极阵列上培养新生大鼠SCN细胞，我们能够记录单个SCN神经元数天或数周的自发动作电位，揭示了放电率的显著昼夜节律。尽管有大量的功能性突触，但同一培养物中神经元表达的昼夜节律并不同步。在可逆阻断神经元放电2.5天后，昼夜节律以不变的阶段重新出现。这些数据表明，SCN包含大量自主的单细胞昼夜节律振荡器，体外形成的突触既不是这些振荡器运行所必需的，也不足以使它们同步。
Within the mammalian hypothalamus, the suprachiasmatic nucleus (SCN) contains a circadian clock for timing of diverse neuronal, endocrine, and behavioral rhythms. By culturing cells from neonatal rat SCN on fixed microelectrode arrays, we have been able to record spontaneous action potentials from individual SCN neurons for days or weeks, revealing prominent circadian rhythms in firing rate. Despite abundant functional synapses, circadian rhythms expressed by neurons in the same culture are not synchronized. After reversible blockade of neuronal firing lasting 2.5 days, circadian firing rhythms re-emerge with unaltered phases. These data suggest that the SCN contains a large population of autonomous, single-cell circadian oscillators, and that synapses formed in vitro are neither necessary for operation of these oscillators nor sufficient for synchronizing them.