Comparative Transcriptomics of Buzura suppressaria (Lepidoptera: Geometridae) Assembled De Novo Yield Insights Into Response After Buzura suppressaria Nuclear Polyhedrosis Virus Infection
Comparative Transcriptomics of Buzura suppressaria (Lepidoptera: Geometridae) Assembled De Novo Yield Insights Into Response After Buzura suppressaria Nuclear Polyhedrosis Virus Infection
复制标题
Buzura压制虫（鳞翅目：尺蛾科）的比较转录组学从头组装，深入了解Buzura压制虫核型多角体病毒感染后的反应。
DOI：
10.1093/jee/tow298
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发表时间：
2017-06-01
影响因子：
2.2
通讯作者：
Wu, Yaojun
中科院分区：
文献类型：
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作者：
Luo, Ji;Zhong, Yating;Wu, Yaojun
地蛾（鳞翅目：尺蛾科）是一种严重危害华南地区桉树的落叶害虫。抑制芽孢核多角体病毒（BsNPV）是一种杆状病毒，能够高特异性和效率地感染抑制芽孢杆菌。使用基于 Illumina 的平台对 BsNPV 感染前后的抑制芽孢杆菌转录组进行测序，以探查病毒感染后抑制芽孢杆菌的差异表达基因 (DEG)。平均而言，生成了大约 5740 万个高质量的干净读数，并从头组装成 69,761 个 unigene。使用NCBI非冗余蛋白、Swiss-Prot、京都基因和基因组百科全书（KEGG）、基因本体论（GO）和Cluster of Orthologous Groups数据库通过NCBI BLAST对unigene进行注释；然后，33,575 个 unigenes (48.1%) 被映射到至少其中一个数据库，4,366 个 unigenes (6.3%) 被映射到所有数据库。差异表达分析显示，在至少一次配对比较中，25,212 个unigenes 上调，22,880 个unigenes 下调。对照与 48 小时相比，GO 或 KEGG 数据库中的其他两对比较具有更多的 DEG，因为受调控基因的数量会随着 BsNPV 感染更多组织而增加，并随着更多组织失效而减少。为了确定抑制芽孢杆菌对 BsNPV 感染的免疫反应，DEG 被注释到 GO 和 KEGG 数据库中。总共有 89 个 GO 类别与免疫反应相关，并且这些 GO 类别注释了 1,007 个 DEG。此外，在所有三组中同时获得了 7 个下调的 DEG 和 37 个上调的 DEG。这些差异基因被认为在整个病毒感染过程中发挥着核心作用。
Buzura suppressaria Guenee (Lepidoptera: Geometridae) is a defoliator that seriously harms eucalyptus trees in South China. Buzura suppressaria nuclear polyhedrosis virus (BsNPV) is a baculovirus that infects B. suppressaria with high specificity and efficiency. Transcriptomes of B. suppressaria were sequenced before and after BsNPV infection using an Illumina-based platform to probe for differentially expressed genes (DEGs) of B. suppressaria after viral infection. On average, similar to 57.4 million high-quality clean reads were generated and assembled de novo into 69,761 unigenes. The NCBI nonredundant protein, Swiss-Prot, Kyoto Encyclopedia of Genes and Genomes (KEGG), Gene ontology (GO), and Cluster of Orthologous Groups databases were used to annotate unigenes through NCBI BLAST; 33,575 unigenes (48.1%) were then mapped to at least one of these databases, and 4,366 unigenes (6.3%) were mapped to all databases. Differential expression analysis showed that 25,212 unigenes were upregulated and 22,880 unigenes were downregulated in at least one pairwise comparison. Control versus 48h had more DEGs than other two pairwise comparisons in either the GO or KEGG database, because the number of regulated gene would increase as BsNPV infected more tissues and would decrease as more tissues were disabled. To ascertain B. suppressaria immune response to BsNPV infection, DEGs were annotated to the GO and KEGG databases. In total, 89 GO categories are related to immune response and 1,007 DEGs are annotated to these GO categories. Furthermore, 7 downregulated DEGs and 37 upregulated were obtained simultaneously in all three groups. These DEGs were considered to possess a central role throughout viral infection.