Zeptomole-sensitivity electrospray ionization - Fourier transform ion cyclotron resonance mass spectrometry of proteins
Zeptomole-sensitivity electrospray ionization - Fourier transform ion cyclotron resonance mass spectrometry of proteins
复制标题
DOI：
10.1021/ac991360b
复制
发表时间：
2000-05-15
影响因子：
7.4
通讯作者：
Smith, RD
中科院分区：
文献类型：
--
作者：
Belov, ME;Gorshkov, MV;Smith, RD
正在开发基于电喷雾电离 (ESI) 和傅里叶变换离子回旋共振质谱 (FTICR-MS) 的超灵敏蛋白质表征方法。配备 ESI 源的 FTICR 质谱仪的灵敏度取决于整体离子传输，其中结合了 FTICR 池中电离概率、传输效率和离子捕获。我们在 3.5 特斯拉 FTICR 质谱仪中实施的开发包括在 ESI 接口中引入和优化新设计的电动离子漏斗、改进四极静电离子导向器接口中的离子束特性以及静电离子导向器的修改。这些进展为分子量范围为 8 至 20 kDa 的蛋白质提供了约 30 zmol（类似于 18 000 个分子）的检测限。
Methods are being developed for ultrasensitive protein characterization based upon electrospray ionization (ESI) with Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS). The sensitivity of a FTICR mass spectrometer equipped with an ESI source depends on the overall ion transmission, which combines the probability of ionization, transmission efficiency, and ion trapping in the FTICR cell. Our developments implemented in a 3.5 tesla FTICR mass spectrometer include introduction and optimization of a newly designed electrodynamic ion funnel in the ESI interface, improving the ion beam characteristics in a quadrupole-electrostatic ion guide interface, and modification of the electrostatic ion guide. These developments provide a detection limit of approximately 30 zmol (similar to 18 000 molecules) for proteins with molecular weights ranging from 8 to 20 kDa.