ERYTHROPOIETIC CELL POPULATION CHANGES DURING HEPATIC PHASE OF ERYTHROPOIESIS IN FOETAL MOUSE
ERYTHROPOIETIC CELL POPULATION CHANGES DURING HEPATIC PHASE OF ERYTHROPOIESIS IN FOETAL MOUSE
复制标题
DOI：
10.1111/j.1365-2184.1969.tb00238.x
复制
发表时间：
1969-01-01
期刊：
影响因子：
--
通讯作者：
FRESHNEY, RI
中科院分区：
文献类型：
--
作者：
PAUL, J;CONKIE, D;FRESHNEY, RI
已经对小鼠中从受精后12.5天开始的肝源性红细胞生成细胞的绝对数量进行了估计。最早期样本中存在直至网织红细胞的所有成熟阶段，但以最不成熟的细胞（原成红细胞和嗜碱性成红细胞）为主;发育后期以更成熟的细胞（正染性成红细胞、网织红细胞和红细胞）为主。肝原性血红蛋白化细胞的数量呈指数增加，群体倍增时间约为8小时，直到受精后约15.5天。然后有一个急剧的转变，倍增时间延长到约2天。在快速增加阶段形成的未成熟细胞血红蛋白化较差;因此血红蛋白的增加滞后于细胞的增加。肝源性血红蛋白化细胞和血红蛋白/标准肝细胞数的形成率的计算显示在15至16天之间达到最大值;这些发现与先前在培养的小鼠胎肝中对血红蛋白合成率的直接观察结果雅阁。
Estimates have been made of the absolute numbers of hepatogenic erythropoietic cells from 12.5 days post fertilization onwards in the mouse. All stages of maturation up to reticulocytes are present in the earliest samples but the least mature cells (proerythroblasts and basophilic erythroblasts) predominate; more mature cells (orthochromatic erythroblasts, reticulocytes and erythrocytes) predominate later in development. The number of hepatogenic haemoglobinized cells increases exponentially with a population doubling time of about 8 hr until about 15.5 days post fertilization. There is then a sharp transition and the doubling time lengthens to about 2 days. The immature cells formed during the rapid phase of increase are poorly haemoglobinized; hence the increase in haemoglobin lags behind that of cells. Calculations of the rates of formation of hepatogenic haemoglobinized cells and haemoglobin per standard number of liver cells show maxima between 15 and 16 days; these findings are in accord with direct observations of rates of haemoglobin synthesis in cultured mouse foetal livers made previously.