Bayesian analysis of new and old malaria parasite DNA sequence data demonstrates the need for more phylogenetic signal to clarify the descent of Plasmodium falciparum
Bayesian analysis of new and old malaria parasite DNA sequence data demonstrates the need for more phylogenetic signal to clarify the descent of Plasmodium falciparum
复制标题
DOI：
10.1007/s00436-007-0499-6
复制
发表时间：
2007-08-01
影响因子：
2
通讯作者：
Kampen, H.
中科院分区：
文献类型：
--
作者：
Hagner, S. C.;Misof, B.;Kampen, H.
在过去的15年里，为了阐明疟疾寄生虫之间的系统发育关系，分子系统学研究已经导致了关于恶性疟原虫起源的两个主要假说：一个是由于较新的宿主转换而支持鸟类起源的；另一个是支持恶性疟原虫及其人类宿主从灵长类祖先进化而来的。本文利用从GenBank获得的大量血孢子虫DNA序列，以及以前在分子系统发育分析中从未考虑过的几种主要疟疾寄生虫的新序列，对三种不同的疟原虫基因，即核18小亚基(SSU)核糖体核糖核酸(RRNA)、线粒体细胞色素b(Cytb)和质粒型酪蛋白分解酶C(ClpC)基因进行了系统发育分析。大多数现代和复杂的基于贝叶斯推断分析的DNA替换模型被用来估计cyt b和ClpC的系统发育树，而18SSU rRNA基因的二级结构则用阶段软件进行了研究。我们的结果表明，目前可用的数据通常在数量上或信息上都不足以解决恶性疟原虫系统发育起源的问题。
Molecular systematic studies published during the last 15 years to clarify the phylogenetic relationships among the malaria parasites have led to two major hypotheses on the descent of Plasmodium falciparum: One supports an avian origin as a result of a relatively recent host switch, and another one favours the evolutionary development of P. falciparum together with its human host from primate ancestors. In this paper, we present phylogenetic analyses of three different Plasmodium genes, the nuclear 18 small sub-unit (SSU) ribosomal ribonucleic acid (rRNA), the mitochondrial cytochrome b (cyt b) and the plastid caseinolytic protease C (ClpC) gene, using numerous haemosporidian parasite DNA sequences obtained from the GenBank as well as several new sequences for major malaria parasites including the avian one Plasmodium cathemerium, which has never been considered in molecular phylogenetic analyses before. Most modern and sophisticated DNA substitution models based on Bayesian inference analysis were applied to estimate the cyt b and ClpC phylogenetic trees, whereas the 18 SSU rRNA gene was examined with regards to its secondary structure using PHASE software. Our results indicate that the data presently available are generally neither sufficient in number nor in information to solve the problem of the phylogenetic origin of P. falciparum.