Programmable and printable Bacillus subtilis biofilms as engineered living materials
Programmable and printable Bacillus subtilis biofilms as engineered living materials
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可编程和可打印的枯草芽孢杆菌生物膜作为工程生活材料
DOI：
10.1038/s41589-018-0169-2
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发表时间：
2019-01-01
影响因子：
14.8
通讯作者：
Zhong, Chao
中科院分区：
文献类型：
--
作者：
Huang, Jiaofang;Liu, Suying;Zhong, Chao
细菌生物膜可以被编程以产生具有自我修复和可进化功能的生物材料。然而，人工生物膜的更广泛应用受到加工性和功能性蛋白质分泌能力的限制。我们描述了一个高度灵活和可调的生活功能材料平台的基础上的TasA淀粉样蛋白机器的细菌枯草芽孢杆菌。我们证明了具有不同功能蛋白或结构域的遗传可编程TasA融合蛋白可以被分泌，并且可以组装成具有可调物理化学性质的不同细胞外纳米结构。我们的工程生物膜具有水凝胶的粘弹性行为，并且可以使用3D打印和微胶囊技术精确地制造成具有多种三维（3D）形状的微结构。值得注意的是，这些持久且对环境敏感的人造生物材料仍然活着，自我再生和功能。这种新的可调平台提供了以前无法实现的性能，为各种生活功能材料在生物材料，生物技术和生物医学具有潜在的应用。
Bacterial biofilms can be programmed to produce living materials with self-healing and evolvable functionalities. However, the wider use of artificial biofilms has been hindered by limitations on processability and functional protein secretion capacity. We describe a highly flexible and tunable living functional materials platform based on the TasA amyloid machinery of the bacterium Bacillus subtilis. We demonstrate that genetically programmable TasA fusion proteins harboring diverse functional proteins or domains can be secreted and can assemble into diverse extracellular nano-architectures with tunable physicochemical properties. Our engineered biofilms have the viscoelastic behaviors of hydrogels and can be precisely fabricated into microstructures having a diversity of three-dimensional (3D) shapes using 3D printing and microencapsulation techniques. Notably, these long-lasting and environmentally responsive fabricated living materials remain alive, self-regenerative, and functional. This new tunable platform offers previously unattainable properties for a variety of living functional materials having potential applications in biomaterials, biotechnology, and biomedicine.