Anti-inflammatory and analgesic effects of paeonol in carrageenan-evoked thermal hyperalgesia
Anti-inflammatory and analgesic effects of paeonol in carrageenan-evoked thermal hyperalgesia
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DOI：
10.1038/sj.bjp.0705360
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发表时间：
2003-07-01
影响因子：
7.3
通讯作者：
Chou, TC
中科院分区：
文献类型：
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作者：
Chou, TC
在角叉菜胶诱发的热痛觉过敏大鼠模型中测试了1-羟丙醇的抗炎和镇痛作用。2.丹皮酚（30、50或100 mg kg（-1），i. p.）剂量依赖性地抑制角叉菜胶诱发的热痛觉过敏。3在角叉菜胶注射后的早期（1.5小时）和晚期（4小时），用丹皮酚处理剂量依赖性地抑制肿瘤坏死因子-α（TNF-α）和白细胞介素-1 β（IL-1 β）的形成，但增强大鼠爪渗出物中IL-10的产生。然而，丹皮酚对IL-6形成的抑制作用仅在晚期观察到。4丹皮酚剂量依赖性地减少大鼠爪渗出物中前列腺素E-2（PGE（2））的形成，在晚期抑制作用更大。然而，仅在后期观察到对硝酸盐生成的抑制（在角叉菜胶注射后4小时），伴随着爪组织中诱导型一氧化氮合酶（iNOS）和环氧合酶-2（考克斯-2）蛋白表达的减弱。5髓过氧化物酶活性升高，这是中性粒细胞浸润的指标，在丹皮酚处理的大鼠中，角叉菜胶注射的爪子中的抗氧化剂也剂量依赖性地减少。在这种炎症模型中的炎症和镇痛作用可能与角叉菜胶注射的大鼠爪中促炎细胞因子NO和PGE（2）的产生减少和抗炎细胞因子IL-10的产生增加有关。此外，在注射角叉菜胶的爪中，iNOS和考克斯-2蛋白表达升高以及中性粒细胞浸润的减弱也可能参与丹皮酚的有益作用。
1 Paeonol was tested for its anti-inflammatory and analgesic effects in a rat model of carrageenan-evoked thermal hyperalgesia. The possible mechanisms involved in these effects were also investigated.2 Pre- and post-treatment with paeonol ( 30, 50 or 100 mg kg(-1), i.p.) dose-dependently inhibited the carrageenan-evoked thermal hyperalgesia.3 Treatment with paeonol dose-dependently inhibited tumour necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta) formation, but enhanced IL-10 production in the rat paw exudates both at the early (1.5 h) and late phase ( 4 h) after carrageenan injection. However, inhibition of IL-6 formation by paeonol was only observed at the late phase.4 Paeonol dose-dependently decreased the formation of prostaglandin E-2 (PGE(2)) in rat paw exudates with a greater inhibition at the late phase. However, inhibition of nitrate generation was observed only during the late phase ( at 4 h after carrageenan injection), accompanied by an attenuation of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) protein expression in paw tissue.5 Elevated myeloperoxidase activity, an indicator of neutrophil infiltration, in carrageenan-injected paws was also dose-dependently reduced in paeonol-treated rats.6 Our results suggest that the mechanisms by which paeonol exerts its anti-inflammatory and analgesic effects in this inflammatory model may be associated with decreased production of proinflammatory cytokines, NO and PGE(2) and increased production of IL-10, an anti-inflammatory cytokine, in carrageenan-injected rat paws. In addition, attenuation of the elevated iNOS and COX-2 protein expression as well as neutrophil infiltration in carrageenan-injected paws may also be involved in the beneficial effects of paeonol.