Interleukin-38 inhibits adipogenesis and inflammatory cytokine production in 3T3-L1 preadipocytes
Interleukin-38 inhibits adipogenesis and inflammatory cytokine production in 3T3-L1 preadipocytes
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DOI：
10.1002/cbin.11428
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发表时间：
2020-07-31
影响因子：
3.9
通讯作者：
Li, Mingcai
中科院分区：
文献类型：
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作者：
Li, Yan;Chen, Sisi;Li, Mingcai
白介素38(IL-38)是具有抗炎活性的IL-1细胞因子家族的新成员。然而，其在体外对脂肪细胞的成脂和炎性细胞因子分泌的影响尚未见报道。为了探讨IL-38是否在体外抑制脂肪生成和炎症，我们在体外培养了脂肪前体细胞3T3-L1，并加入或不加IL-38。油红O染色检测3T3-L1细胞内脂滴的形态和大小。实时定量聚合酶链式反应检测3T3-L1细胞GATA结合蛋白-3(GATA-3)、葡萄糖转运蛋白4(GLUT4)、过氧化物酶体增殖物相关受体γ2、IL-1β、IL-6和单核细胞趋化蛋白-1(MCP-1)的mRNA表达水平，酶联免疫吸附试验检测3T3-L1细胞培养上清液中IL-6、IL-1β和MCP-1的含量。IL-38可显著减少3T3-L1细胞的脂滴数量。IL-38还可促进3T3-L1细胞GATA-3和GLUT4mRNA的表达，抑制IL-1β、IL-6和MCP-1的分泌。结论：IL-38可抑制人脂肪细胞分化，抑制3T3-L1细胞产生炎性细胞因子。
Interleukin-38 (IL-38) is a novel member of the IL-1 cytokine family with anti-inflammatory activity. However, its effect on adipogenesis and inflammatory cytokines secretion of adipocytes in vitro has not been reported. To address whether IL-38 inhibits adipogenesis and inflammation in vitro, adipose precursor 3T3-L1 cells were cultured with or without IL-38. The morphology and size of lipid droplets in 3T3-L1 cells were measured by Oil red O staining. The mRNA expression levels of GATA-binding protein-3 (GATA-3), glucose transporter type 4 (GLUT4), peroxisome proliferator-associated receptor gamma 2, IL-1 beta, IL-6, and monocyte chemoattractant protein-1 (MCP-1) in 3T3-L1 cells were detected by real-time PCR, The contents of IL-6, IL-1 beta, and MCP-1 in 3T3-L1 cell medium supernatants were determined by enzyme-linked immunosorbent assay. IL-38 significantly decreased the number of lipid droplets in 3T3-L1 cells. IL-38 also increased GATA-3 and GLUT4 mRNA expression and inhibited IL-1 beta, IL-6, and MCP-1 secretion by 3T3-L1 cells. It is concluded that IL-38 can inhibit the differentiation of human adipocytes and inflammatory cytokine production by 3T3-L1 cells.