Cytocompatibility and Bone-Formation Potential of Se-Coated 316L Stainless Steel with Nano-Pit Arrays
Cytocompatibility and Bone-Formation Potential of Se-Coated 316L Stainless Steel with Nano-Pit Arrays
复制标题
具有纳米坑阵列的 Se 涂层 316L 不锈钢的细胞相容性和骨形成潜力
DOI：
10.1166/jbn.2018.2526
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发表时间：
2018-04-01
影响因子：
2.9
通讯作者：
Ni, Siyu
中科院分区：
文献类型：
--
作者：
Hu, Haoran;Cui, Ran;Ni, Siyu
316L不锈钢由于其机械性能、耐腐蚀性和相对较低的价格，在关节置换和骨科手术中仍被广泛应用。为了提高316L不锈钢的表面性能、生物相容性和骨结合能力，本研究采用电化学氧化和纳米涂层的方法制备了纳米点阵镀Se的316L不锈钢。通过场发射扫描显微镜(FESEM)、能量色散X射线能谱(EDS)、X射线光电子能谱(XPS)和Se释放研究对改性后的316L不锈钢进行了测试。研究结果表明，纳米坑阵列的直径为50 nm，涂层主要由元素Se组成，并具有缓释作用。用大鼠骨髓间充质干细胞(RBMSCs)体外实验和体内动物实验评价样品的生物学反应。FESEM、CCK-8检测、免疫荧光显微镜(IF)和碱性磷酸酶(ALP)体外活性测定以及体内新骨形成实验表明，改性后的316L不锈钢具有更强的细胞黏附、增殖和成骨活性，具有良好的细胞相容性和成骨分化能力。更重要的是，Se涂层可以上调OPN、RUNX-2和ALP的基因表达，表明纳米Se涂层316L不锈钢纳米坑阵列是一种很有前途的生物医学材料，可用于骨科或牙科临床植入物。
316L stainless steel is still widely applied in joint replacement and orthopedic surgery due to its mechanical properties, corrosion resistance and relatively low price. In this study, electrochemical oxidation and nanoscale coating were used to fabricate Se-coated 316L stainless steel with nano-pit arrays to enhance its surface characteristics, biocompatibility and osseointegration ability. The modified 316L stainless steel was tested via field emission scanning microscopy (FESEM), energy-dispersive X-ray spectrometry (EDS), X-ray photoelectron spectroscopy (XPS) and Se release studies. The results of this study showed that the nano-pit arrays were 50 nm in diameter and that the Se coating consisted primarily of elemental Se and exhibited sustained release. The biological response of the samples was evaluated using in vitro rat bone marrow mesenchymal stem cell (rBMSCs) experiments and in vivo animal experiments. The modified 316L stainless steel displays enhanced abilities of cell adhesion, proliferation and osteogenic activity, as shown by FESEM, CCK-8 assay, immunofluorescence microscopy (IF) and alkaline phosphatase (ALP) activity assay in vitro and additional new bone formation in vivo, indicating its outstanding cytocompatibility and osteogenic differentiation ability. More importantly, the Se coating can upregulate gene expression of OPN, RUNX-2 and ALP, indicating that the nano-Se-coated 316L stainless steel with nano-pit arrays is a promising biomedical material for implants in orthopedic or dental clinic applications.