The role of BMP-4 and GATA-2 in the induction and differentiation of hematopoietic mesoderm in Xenopus laevis
The role of BMP-4 and GATA-2 in the induction and differentiation of hematopoietic mesoderm in Xenopus laevis
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DOI：
10.1182/blood.v88.6.1965.bloodjournal8861965
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发表时间：
1996-09-15
期刊：
影响因子：
20.3
通讯作者：
Zon, LI
中科院分区：
文献类型：
--
作者：
Maeno, M;Mead, PE;Zon, LI
脊椎动物胚胎血液的形成是由参与背腹模式和中胚层诱导的因素调节的。gata结合转录因子是正常造血所必需的，在原肠胚形成过程中，当腹侧中胚层（VM）被诱导形成血液时表达。基于骨形态发生蛋白（bone morphogenetic protein, BMP-4）是一种有效的腹化因子和造血组织诱导剂，我们假设BMP-4可以诱导或激活GATA-2。我们发现BMP-4可以刺激GATA-5的表达，并且BMP-4显性负受体的表达可以抑制BMP-4在腹侧中胚层诱导GATA-2， GATA-2在腹侧中胚层过表达导致珠蛋白产生增加，GATA-2在靠近腹侧中胚层的原始外胚层强制表达也刺激珠蛋白的表达。我们的研究结果表明，BMP-4和GATA-2可以在两个相邻的胚层中发挥作用，即中胚层和外胚层，参与胚胎发生过程中血细胞的形成。(C) 1996年由美国血液病学会出版。
Vertebrate embryonic blood formation is regulated by factors that participate in dorsal-ventral patterning and mesoderm induction. The GATA-binding transcription factors are required for normal hematopoiesis and are expressed during gastrulation when ventral mesoderm (VM) is induced to form blood. Based on the recent demonstration that bone morphogenetic protein (BMP-4) is a potent ventralizing factor and inducer of hematopoietic tissue, we hypothesized that GATA-2 could be induced or activated by BMP-4. Here we demonstrate that BMP-4 can stimulate GATA-5 expression, and that expression of a dominant negative BMP-4 receptor can suppress GATA-2 induction by BMP-4 in ventral mesoderm, Over-expression of GATA-2 in ventral mesoderm leads to increased globin production and forced expression of GATA-2 in primitive ectoderm adjacent to ventral mesoderm also stimulates globin expression. Our results suggest that BMP-4 and GATA-2 can function in two adjacent germ layers, mesoderm and ectoderm, to participate in blood cell formation during embryogenesis. (C) 1996 by The American Society of Hematology.