Identification and characterization of a gene encoding a putative mouse Rho GTPase activating protein gene 8, Arhgap8.
Identification and characterization of a gene encoding a putative mouse Rho GTPase activating protein gene 8, Arhgap8.
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编码假定的小鼠 Rho GTPase 激活蛋白基因 8（Arhgap8）的基因的鉴定和表征。
DOI：
10.1016/s0378-1119(02)01143-5
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发表时间：
2003
期刊：
影响因子：
3.5
通讯作者：
Popescu,NicholasC
中科院分区：
文献类型：
--
作者：
Shan,Zhihong;Haaf,Thomas;Popescu,NicholasC
Rho GTP酶激活蛋白促进Rho家族蛋白的内在GTP水解活性。我们从小鼠中分离出了与人类Rho GTPase激活蛋白8 （ARHGAP8）同源的基因。该开放阅读框编码一个与人类ARHGAP8 n端结构域高度同源的387个氨基酸的肽。辐射杂交定位和荧光原位杂交都将该基因定位在小鼠染色体15E上。全长23 kb的Arhgap8基因组序列由8个外显子和7个内含子组成。Northern blot和RT-PCR分析显示，约1.9 kb的转录本在成年小鼠的各种组织中普遍表达，在肾脏中表达尤为强烈。
Rho GTPase activating proteins promote the intrinsic GTP hydrolysis activity of Rho family proteins. We isolated a putative mouse ortholog of the human Rho GTPase activating protein 8, ARHGAP8. The open reading frame encodes a peptide of 387 amino acids with high homology to human ARHGAP8 in its N-terminal domain. Both radiation hybrid mapping and fluorescent in situ hybridization localized the gene to mouse chromosome 15E. The 23 kb genomic Arhgap8 sequence consists of eight exons and seven introns. Northern blot and RT-PCR analyses showed that a transcript of approximately 1.9 kb is ubiquitously expressed in various adult mouse tissues with particularly strong expression in kidney.