Evaluation of Streptomyces sp strain g10 for suppression of Fusarium wilt and rhizosphere colonization in pot-grown banana plantlets
Evaluation of Streptomyces sp strain g10 for suppression of Fusarium wilt and rhizosphere colonization in pot-grown banana plantlets
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DOI：
10.1007/s10295-004-0199-5
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发表时间：
2005-01-01
影响因子：
3.4
通讯作者：
Goodfellow, M
中科院分区：
文献类型：
--
作者：
Getha, K;Vikineswary, S;Goodfellow, M
链霉菌属。菌株g10对Fusarium oxysporum f.sp表现出强烈的拮抗作用。 cubense (Foc) 通过产生细胞外抗真菌代谢物在平板测定中进行比赛 1、2 和 4。用菌株 g10 悬浮液 (10(8) cfu/ml) 处理 4 周龄组织培养来源的“Novaria”香蕉幼苗的种植孔和根部，当幼苗接种 10(4) 孢子/ml Foc race 4 时，显着（P < 0.05）降低枯萎严重程度。叶子症状 (LSI) 和根茎变色 (RDI) 的最终疾病严重程度指数降低约与未处理的小植株相比，经 g10 菌株处理的小植株分别为 47% 和 53%。当苗接种较高浓度（10（6）孢子/ml）的Foc小种4时，发病率降低并不显着（P < 0.05）。与拮抗剂改良土壤后的第2周相比，在第3周结束时，菌株g10的根际种群显着（P = 0.05）增加2倍以上。尽管水平有所下降，但第 6 周结束时的根际种群数量仍比 2 周后检测到的水平高出近 2 倍。相比之下，无根种群显着下降（P = 0.05），与 2 周后检测到的水平相比，6 周后下降了近 4 倍。在菌株 g10 改良土壤中生长的小植株中，既没有观察到生长抑制作用，也没有观察到生长刺激作用。
Streptomyces sp. strain g10 exhibited strong antagonism towards Fusarium oxysporum f.sp. cubense (Foc) races 1, 2 and 4 in plate assays by producing extracellular antifungal metabolites. Treating the planting hole and roots of 4-week-old tissue-culture-derived 'Novaria' banana plantlets with strain g10 suspension (10(8) cfu/ml), significantly (P < 0.05) reduced wilt severity when the plantlets were inoculated with 10(4) spores/ml Foc race 4. The final disease severity index for leaf symptom (LSI) and rhizome discoloration (RDI) was reduced about 47 and 53%, respectively, in strain g10-treated plantlets compared to untreated plantlets. Reduction in disease incidence was not significant (P < 0.05) when plantlets were inoculated with a higher concentration (10(6) spores/ml) of Foc race 4. Rhizosphere population of strain g10 showed significant (P = 0.05) increase of more than 2-fold at the end of the 3rd week compared to the 2nd week after soil amendment with the antagonist. Although the level dropped, the rhizosphere population at the end of the 6th week was still nearly 2-fold higher than the level detected after 2 weeks. In contrast, the root-free population declined significantly (P = 0.05), nearly 4-fold after 6 weeks when compared to the level detected after 2 weeks. Neither growth-inhibiting nor growth-stimulating effects were observed in plantlets grown in strain g10-amended soil.