More sex-determination mutants of Caenorhabditis elegans.
More sex-determination mutants of Caenorhabditis elegans.
复制标题
秀丽隐杆线虫的更多性别决定突变体。
作者：
J. Hodgkin
秀丽隐杆线虫的性别决定受X染色体：常染色体比例控制，即2A;XX动物是雌雄同体，2A;XO动物是雄性。已经开发了分离转化为雌雄同体的2A;XO动物的程序。九个突变导致这种转变已经获得：八个是隐性的，所有这些都属于一个新的常染色体互补组，她-1 V。剩余的突变（她-2）是显性的，并具有遗传图谱的位置类似的tra-1 III。tra-1的隐性突变导致逆转，将2A;XX动物转化为雄性。因此，her-2突变可能导致tra-1的组成型表达。HER-1突变对XX动物没有影响，但HER-2突变除了对XO动物的性表型有影响外，还阻止了XX和XO动物的精子产生。tra和her基因间的上位关系被用来推导这些基因在控制性别决定中的作用模型。
Sex determination in Caenorhabditis elegans is controlled by the X chromosome : autosome ratio, i.e. 2A;XX animals are hermaphrodite, and 2A;XO animals are male. A procedure for isolating 2A;XO animals that are transformed in;to hermaphrodites has been developed. Nine mutations causing this transformation have been obtained: eight are recessive, and all of these fall into a new autosomal complementation group, her-1 V. The remaining mutation (her-2) is dominant and has a genetic map location similar to that of tra-1 III. Recessive mutations of tra-1 cause the reverse transformation, transforming 2A;XX animals into males. Therefore, the her-2 mutation may result in constitutive expression of tra-1. Mutations in her-1 are without effect on XX animals, but the her-2 mutation prevents sperm production in both XX and XO animals, in addition to its effect on the sexual phenotype of XO animals. The epistatic relationships between tra and her genes are used to deduce a model for the action of these genes in controlling sex determination.