Effects of Herb-Partitioned Moxibustion on the miRNA Expression Profiles in Colon from Rats with DSS-Induced Ulcerative Colitis.
Effects of Herb-Partitioned Moxibustion on the miRNA Expression Profiles in Colon from Rats with DSS-Induced Ulcerative Colitis.
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隔药灸对 DSS 溃疡性结肠炎大鼠结肠 miRNA 表达谱的影响
DOI：
10.1155/2017/1767301
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发表时间：
2017
期刊：
影响因子：
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通讯作者：
Wu HG
中科院分区：
文献类型：
--
作者：
Huang Y;Ma Z;Cui YH;Dong HS;Zhao JM;Dou CZ;Liu HR;Li J;Wu HG
Objective.本研究从miRNA的角度探讨隔药灸对葡聚糖硫酸钠（DSS-）诱导的溃疡性结肠炎（UC）的作用机制。方法.将大鼠随机分为正常对照组（NC）、UC模型组（UC）和隔药灸组（UCHM）。UC和UCHM组给予4%DSS 7天。UCHM组在天枢（双侧，ST 25）接受HM。观察HM对UC的影响，并分析结肠组织中miRNA的表达谱。结果与UC组相比，UCHM组第14天的体重显著增加（P <0. 001）; UCHM组的肉眼结肠损伤评分和显微组织病理学评分均降低（P <0. 05）; UCHM组差异表达的miRNAs有15个。UC中miR-184和miR-490- 5 p表达水平的变化通过HM干预逆转。经qRT-PCR验证，两种miRNAs的表达趋势与测序结果一致。结论HM在ST 25可能调节miR-184和miR-490- 5 p的表达，作用于其靶基因的转录以调节炎症信号通路，并减轻DSS诱导的UC大鼠结肠中的炎症和组织损伤。
Objective. This study explored the mechanism of herb-partitioned moxibustion (HM) on dextran sulfate sodium- (DSS-) induced ulcerative colitis (UC) from the miRNA perspective. Methods. Rats were randomly divided into 3 groups [normal control (NC) group, UC model (UC) group, and herb-partitioned moxibustion (UCHM) group]. The UC and UCHM groups were administered 4% DSS for 7 days. The UCHM group received HM at the Tianshu (bilateral, ST25). The effect of HM on UC was observed and the miRNA expression profile in the colon tissues was analyzed. Results. Compared with the UC group, the body weights were significantly higher in the UCHM group on day 14 (P < 0.001); the macroscopic colon injury scores and microscopic histopathology scores in the UCHM group decreased (P < 0.05); and there were 15 differentially expressed miRNAs in the UCHM group. The changes in miR-184 and miR-490-5p expression levels on the UC were reversed by HM intervention. Validation using qRT-PCR showed that two miRNAs expression trend was consistent with the sequencing results. Conclusion. HM at ST25 might regulate miR-184 and miR-490-5p expression, act on the transcription of their target genes to regulate inflammatory signaling pathways, and attenuate inflammation and tissue injury in the colons of rats with DSS-induced UC.