Δ6-Desaturase from Mortierella alpina: cDNA cloning, expression, and phylogenetic analysis
Δ6-Desaturase from Mortierella alpina: cDNA cloning, expression, and phylogenetic analysis
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DOI：
10.1007/s10529-011-0650-4
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发表时间：
2011-06
影响因子：
2.7
通讯作者：
Jianmin Liu;Dianrong Li;Yongtai Yin;Hao Wang;Maoteng Li;Longjiang Yu
中科院分区：
文献类型：
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作者：
Jianmin Liu;Dianrong Li;Yongtai Yin;Hao Wang;Maoteng Li;Longjiang Yu
从高山被孢霉（Mortierella alpinaW 15）中克隆了Δ6-脱氢酶基因的开放阅读框架，并将其克隆到pPIC 3.5K载体中。将该载体转化到毕赤酵母GS115中，用甲醇诱导表达。Δ6-去饱和酶在P. pastorisGS 115催化亚油酸转化为γ-亚麻酸，但不催化α-亚麻酸转化为十八碳四烯酸。结果表明，Δ6-去饱和酶基因来自mM. alpinaW 15在不同生物体中具有底物特异性。系统发育分析表明，Δ6-去饱和酶基因可分为4个单系群。这为进一步研究Δ6-去饱和酶在脂肪酸代谢中的功能及其三维结构奠定了基础。
The open reading frame of the Δ6-desaturase gene was isolated fromMortierella alpinaW15 and the gene was cloned into a pPIC3.5K vector. The vector was transformed intoPichia pastorisGS115 and expression was induced with methanol. The Δ6-desaturase expressed inP. pastorisGS115 catalyzed the conversion of linoleic acid to γ-linolenic acid but not the conversion of α-linolenic acid to octadecatetraenoic acid. The results indicate that the Δ6-desaturase gene fromM. alpinaW15 has substrate specificity in different organisms. Phylogenetic analysis revealed that Δ6-desaturase genes can be divided into four monophyletic groups. This work paves the way for further study of the functions of Δ6-desaturase in fatty acid metabolism and its three-dimensional structure.