Epidermal growth factor (EGF) increases the in vitro invasion, motility and adhesion interactions of the primary renal carcinoma cell line, A704
Epidermal growth factor (EGF) increases the in vitro invasion, motility and adhesion interactions of the primary renal carcinoma cell line, A704
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DOI：
10.1016/0959-8049(96)00207-9
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发表时间：
1996-10-01
影响因子：
8.4
通讯作者：
Haites, NE
中科院分区：
文献类型：
--
作者：
Price, JT;Wilson, HM;Haites, NE
转移是一个多步骤的过程，涉及肿瘤细胞侵袭、运动和粘附能力的改变。本研究检测了 EGF 对原代肾腺癌细胞系 A704 的体外侵袭、运动和粘附的影响。 EGF (40 ng/ml) 刺激肿瘤细胞 24 小时可增加体外侵袭 (P = 0.040) 和运动性 (P = 0.039)。检查纤连蛋白、层粘连蛋白、IV 型胶原蛋白和三种细胞外基质成分 1:1:1 混合物的细胞粘附情况。 EGF (40 ng/ml) 刺激后，纤连蛋白 (P = 0.022) 和 IV 型胶原 (P = 0.026) 的粘附力显着降低，但细胞外基质成分 1:1:1 混合的粘附力增加 (P = 0.022)。测量时，细胞条件培养基中存在的 92 kDa 基质金属蛋白酶 (MMP-9) 在用 EGF (40 ng/ml) 刺激 24 小时后也有所增加。因此，EGF可以调节A704细胞系的体外侵袭、运动、粘附和基质金属蛋白酶的产生，随后可能在一些肾癌的转移潜力中发挥作用。版权所有 (C) 1996 爱思唯尔科学有限公司
Metastasis is a multistep process that involves alterations in a tumour cell's invasion, motility and adhesive capabilities. This study examined the effect of EGF on the in vitro invasion, motility and adhesion of the primary renal adenocarcinoma cell line, A704. Stimulation of the tumour cells by EGF (40 ng/ml) for a period of 24 h increased the in vitro invasion (P = 0.040) and motility (P = 0.039). Cell adhesion was examined on fibronectin, laminin, collagen IV and a 1:1:1 mix of the three extracellular matrix components. After EGF (40 ng/ml) stimulation, adhesion was significantly decreased on fibronectin (P = 0.022) and collagen type IV (P = 0.026), but increased on the 1:1:1 mix of extracellular matrix components (P = 0.022). The 92 kDa matrix metalloproteinase (MMP-9) present in the cell-conditioned medium was also increased after a 24 h stimulation with EGF (40 ng/ml) when measured. Hence, EGF can modulate the in vitro invasion, motility, adhesiveness and matrix metalloproteinase production in the A704 cell line, and subsequently may have a role in the metastatic potential of some renal carcinomas. Copyright (C) 1996 Elsevier Science Ltd