Lysosome-associated membrane protein-1 (LAMP-1) is the melanocyte vesicular membrane glycoprotein band II.
Lysosome-associated membrane protein-1 (LAMP-1) is the melanocyte vesicular membrane glycoprotein band II.
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溶酶体相关膜蛋白-1 (LAMP-1) 是黑素细胞囊泡膜糖蛋白带 II。
DOI：
10.1111/1523-1747.ep12462775
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Orlow,SJ
中科院分区：
文献类型：
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作者：
Zhou,BK;Boissy,RE;Pifko-Hirst,S;Moran,DJ;Orlow,SJ
包被囊泡在黑素生成过程中起着关键作用。抗小鼠黑色素瘤细胞的包被囊泡组分产生的抗血清识别两种主要的糖蛋白抗原，条带I（47-55 kd）和条带II（90-120 kd）。我们通过以下标准证明带II是溶酶体相关膜蛋白1（lysosome-associated membrane protein 1，LAMP-1）：1）LAMP-1的分子量和丰度在不同组织中不同，但总是与带II相同; 2）带II和LAMP-1在蔗糖梯度沉降研究中共迁移; 3）用抗囊泡血清免疫耗竭细胞提取物去除所有LAMP-1; 4）抗囊泡血清免疫分离的完整细胞器含有带II和LAMP 1。我们的研究结果进一步证实了长期怀疑黑素体和细胞器的溶酶体谱系之间的关系。
Coated vesicles play a critical role in the process of melanogenesis. Antisera raised against a coated vesicle fraction from mouse melanoma cells recognize two major glycoprotein antigens, band I (47-55 kd) and band II (90-120 kd). We demonstrate that band II is lysosome-associated membrane protein 1 (LAMP-1) by the following criteria: 1) the molecular weight and abundance of LAMP-1 varies among tissues but is always identical to that of band II; 2) band II and LAMP-1 co-migrate in sucrose gradient sedimentation studies; 3) immunodepletion of cell extracts with antivesicle serum removes all LAMP-1; and 4) intact organelles immunoisolated with antivesicle serum contain band II and LAMP1. Our results further confirm the long-suspected relationship between melanosomes and the lysosomal lineage of organelles.