The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast
The isolation of Pi1, an allele at the Pik locus which confers broad spectrum resistance to rice blast
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DOI：
10.1007/s00122-012-1894-7
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发表时间：
2012-09-01
影响因子：
5.4
通讯作者：
Pan, Qinghua
中科院分区：
文献类型：
--
作者：
Hua, Lixia;Wu, Jianzhong;Pan, Qinghua
我们报告的分离Pi1，一个基因赋予广谱抗稻瘟病（Magnaporthe稻瘟病）。使用损失和增益的功能的方法，我们证明，Pi1是一个等位基因在Pik基因座。与该基因座上的其他等位基因一样，Pi1由两个基因组成。一个功能性核苷酸多态性（FNP）被确定，允许区分Pi1从其他Pik等位基因和其他非Pik基因。一个广泛的种质资源调查，使用这种FNP揭示，Pi1是一种罕见的等位基因在种质资源的集合和一个赋予持久的抗性广谱的病原体分离物。
We report the isolation of Pi1, a gene conferring broad-spectrum resistance to rice blast (Magnaporthe oryzae). Using loss- and gain-of-function approaches, we demonstrate that Pi1 is an allele at the Pik locus. Like other alleles at this locus, Pi1 consists of two genes. A functional nucleotide polymorphism (FNP) was identified that allows differentiation of Pi1 from other Pik alleles and other non-Pik genes. A extensive germplasm survey using this FNP reveals that Pi1 is a rare allele in germplasm collections and one that has conferred durable resistance to a broad spectrum of pathogen isolates.