Critical Role of VEGF as a Direct Regulator of Photoreceptor Function.
Critical Role of VEGF as a Direct Regulator of Photoreceptor Function.
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VEGF 作为光感受器功能直接调节剂的关键作用。
DOI：
10.1007/978-3-031-27681-1_71
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发表时间：
2023
影响因子：
--
通讯作者：
Le,Yun-Zheng
中科院分区：
文献类型：
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作者：
Hu,Jianyan;Zhu,Meili;Li,Dai;Wu,Qiang;Le,Yun-Zheng
血管内皮生长因子（VEGF或VEGF-A）是糖尿病和缺氧性血视网膜屏障（BRB）疾病的主要致病因子，已被证明是外周和中枢神经系统中神经元的直接功能调节剂。为了确定VEGF是否在调节视网膜神经元功能中起直接作用，我们建立了特定的实验程序，并利用视网膜电图（ERG）检查了重组VEGF（rVEGF）对小鼠感光细胞功能的影响。在我们的情况下，rVEGF引起暗适应野生型（WT）小鼠中暗适应ERG a波和b波振幅以及明视ERG b波振幅以剂量依赖性方式显著降低，在黑暗中玻璃体内递送rVEGF后不久。然而，在成年秋田糖尿病小鼠中，rVEGF对感光细胞功能的作用被抵消。我们的数据有力地表明，VEGF是光感受器功能的直接调节剂，并且VEGF上调对糖尿病诱导的光感受器功能降低有显著贡献。在本章中，我们将讨论相关的背景，关键的实验程序和结果，以及我们的工作的临床意义。
Vascular endothelial growth factor (VEGF or VEGF-A), a major pathogenic factor for diabetic and hypoxic blood–retina barrier (BRB) diseases, has been shown to act as a direct functional regulator for neurons in the peripheral and central nerve systems. To determine if VEGF plays a direct role in regulating retinal neuronal function, we established specific experimental procedures and examined the effect of recombinant VEGF (rVEGF) on photoreceptor function with electroretinography (ERG) in mice. In our case, rVEGF caused a significant reduction of scotopic ERG a-wave and b-wave amplitudes and photopic ERG b-wave amplitudes in a dose-dependent manner in dark-adapted wild-type (WT) mice, shortly after the intravitreal delivery of rVEGF in dark. However, the effect of rVEGF on photoreceptor function was nullified in adult Akita diabetic mice. Our data strongly suggest that VEGF is a direct regulator of photoreceptor function and VEGF upregulation contributes significantly to the diabetes-induced reduction of photoreceptor function. In this chapter, we will discuss the relevant background, key experimental procedures and results, and clinical significance of our work.