Exocytosis in neuroendocrine cells: New tasks for actin
Exocytosis in neuroendocrine cells: New tasks for actin
复制标题
DOI：
10.1016/j.bbamcr.2006.09.004
复制
发表时间：
2006-11-01
影响因子：
5.1
通讯作者：
Gasman, Stephane
中科院分区：
文献类型：
--
作者：
Malacombe, Magali;Bader, Marie-France;Gasman, Stephane
大多数分泌细胞响应细胞表面受体刺激而经历钙调节的胞吐作用，显示出致密的质膜下肌动蛋白网络，该网络在胞吐过程中被重塑。这篇综述总结了皮质肌动蛋白细胞骨架在胞吐作用中的作用的新见解。许多早期发现支持肌动蛋白物理屏障模型，即皮质肌动蛋白丝的瞬时解聚允许囊泡进入质膜上适当的对接和融合位点。另一方面，我们实验室和其他实验室的数据现在表明肌动蛋白聚合在胞吐过程中也发挥着积极作用。在这里，我们讨论了肌动蛋白细胞骨架在胞吐过程的每个主要步骤中的潜在功能，包括分泌颗粒与质膜的招募、对接和融合。此外，我们还提出了肌动蛋白结合蛋白，它们可能将肌动蛋白组织与胞吐途径中的钙信号联系起来。本综述中引用的结果主要来自对肾上腺髓质嗜铬细胞的研究，这种细胞模型多年来一直是有关胞吐作用分子机制的信息来源。 (c) 2006 Elsevier B.V. 保留所有权利。
Most secretory cells undergoing calcium-regulated exocytosis in response to cell surface receptor stimulation display a dense subplasmalemmal actin network, which is remodeled during the exocytotic process. This review summarizes new insights into the role of the cortical actin cytoskeleton in exocytosis. Many earlier findings support the actin-physical-barrier model whereby transient depolymerization of cortical actin filaments permits vesicles to gain access to their appropriate docking and fusion sites at the plasma membrane. On the other hand, data from our laboratory and others now indicate that actin polymerization also plays a positive role in the exocytotic process. Here, we discuss the potential functions attributed to the actin cytoskeleton at each major step of the exocytotic process, including recruitment, docking and fusion of secretory granules with the plasma membrane. Moreover, we present actin-binding proteins, which are likely to link actin organization to calcium signals along the exocytotic pathway. The results cited in this review are derived primarily from investigations of the adrenal medullary chromaffin cell, a cell model that is since many years a source of information concerning the molecular machinery underlying exocytosis. (c) 2006 Elsevier B.V. All rights reserved.