High-density lipoproteins (HDL) composition and function in preeclampsia
High-density lipoproteins (HDL) composition and function in preeclampsia
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DOI：
10.1007/s00404-018-4824-3
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发表时间：
2018-08-01
影响因子：
2.6
通讯作者：
Zitman-Gal，Tali
中科院分区：
文献类型：
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作者：
Einbinder，Yael;Biron-Shental，Tal;Zitman-Gal，Tali
目的评价子痫前期（PE）妇女高密度脂蛋白（HDL）/胆固醇（包括载脂蛋白A-1（ApoA 1）和对氧磷酶1（PON 1））的性质，它们都是心血管风险的阴性预测因子; PE是一种多系统疾病，其特征是在妊娠后半期出现高血压和蛋白尿或其他终末器官功能障碍。先兆子痫与以后心血管疾病的风险增加有关。高密度脂蛋白，胆固醇水平和发展动脉粥样硬化性心血管疾病的风险之间的负相关性是公认的。MethodsTwenty五个孕妇[19与PE和6与正常妊娠（NP）]被招募在入院分娩。从血液样品中分离HDL。检测血清中PON 1活性和HDL含量。采用体外培养的内皮细胞模型，观察高密度脂蛋白（HDL）对血管细胞粘附分子-1（VCAM-1）转录和内皮型一氧化氮合酶（eNOS）mRNA表达的影响。结果PE组PON 1活性（单位/ml血清）低于正常妊娠组（6.51 ± 0.73vs.9.98 ± 0.54，P= 0.015）。与NP-HDL相比，PE-HDL释放的ApoA 1增加（3.54 ± 0.72 vs. 0.89 ± 0.35;P= 0.01）。与NP-HDL相比，PE-HDL在TNF-α刺激的内皮细胞上VCAM-1 mRNA表达增加，eNOS mRNA表达减少。这一过程与HDL直径增加有关，表明HDL抗氧化活性受损。这些变化可能导致PE女性的长期心血管风险更高。
PurposeTo evaluate (a) the properties of high-density lipoproteins (HDL)/cholesterol, which include apolipoprotein A-1 (ApoA1) and paraoxonase1 (PON1), both are negative predictors of cardiovascular risk and (b) HDL function, among women with preeclampsia (PE). PE is a multi-system disorder, characterized by onset of hypertension and proteinuria or other end-organ dysfunction in the second half of pregnancy. Preeclampsia is associated with increased risk for later cardiovascular disease. The inverse association between HDL, cholesterol levels and the risk of developing atherosclerotic cardiovascular disease is well-established.MethodsTwenty-five pregnant women [19 with PE and 6 with normal pregnancy (NP)] were recruited during admission for delivery. HDL was isolated from blood samples. PON1 activity and HDL were analyzed. An in vitro model of endothelial cells was used to evaluate the effect of HDL on the transcription response of vascular cell adhesion molecule-1 (VCAM-1) and endothelial nitric oxide synthase (eNOS) mRNA expression.ResultsPON1 activity (units/ml serum) was lower in the PE group compared to normal pregnancy (NP) (6.51 ± 0.73 vs. 9.98 ± 0.54;P= 0.015). Increased ApoA1 was released from PE-HDL as compared to NP-HDL (3.54 ± 0.72 vs. 0.89 ± 0.35;P= 0.01). PE-HDL exhibited increased VCAM-1 mRNA expression and decreased eNOS mRNA expression on TNF-α stimulated endothelial cells as compared to NP-HDL.ConclusionsHDL from women with PE reduced PON1 activity and increased ApoA1 release from HDL particles. This process was associated with increased HDL diameter, suggesting impaired HDL anti-oxidant activity. These changes might contribute to higher long-term cardiovascular risks among women with PE.