The major acute phase proteins of bovine milk in a commercial dairy herd
The major acute phase proteins of bovine milk in a commercial dairy herd
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商业奶牛群中牛奶的主要急性期蛋白
DOI：
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发表时间：
2015
影响因子：
2.6
通讯作者：
P. Eckersall
中科院分区：
文献类型：
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作者：
F. Thomas;M. Waterston;P. Hastie;T. Parkin;H. Haining;P. Eckersall
乳汁急性时相蛋白(APP)已被发现，并有望成为乳房炎的生物标志物。然而，为了确认它们在乳房炎诊断中的益处，有必要对生产牛群中它们的特征进行分析。对来自某商业奶牛场的54份复合乳样(采自同一容器的奶牛乳房各功能部位的乳汁)进行了乳结合珠蛋白(HP)、乳房相关血清淀粉样蛋白A3(M-SAA3)和C反应蛋白(CRP)的测定。建立了检测奶牛乳汁Hp的酶联免疫吸附试验(EL ISA)方法，并用商品化试剂盒分别检测了M- = 和CRP值。结果复合乳HP范围为0.4~55μg/ml，中位数为3.5μg/ml，复合乳M-SAA3范围为0.6~50μg/ml，中位数为1.2μg/ml，CRP1.8~173 ng/ml，中位数为24.6 ng/ml。0.009)和奇偶与HP(P <； 0.009)，但M-SAA3与鳞癌、M-SAA3与HP、M-SAA3与C反应蛋白或M-SAA3与奇偶之间不存在差异。乳中C反应蛋白与乳样中脂肪百分比(P = 0.002)和蛋白质百分比(P = 0.001)相关。鳞状细胞癌与M-SAA3和CRP之间缺乏相关性可能是由于这些APP对乳腺内感染比SCC更敏感。1/4奶样Hp的检出范围为0.4~420g/ml，中位数为3.6g/μ，其中92%的乳样中Hp、M-μ和CRP值均在20μg/ml以下。在用幽门螺杆菌诊断乳腺炎时，产次被认为是一个可能的混杂因素。APP、HP、M-SAA3、CRP作为乳房炎症的代用品或补充SCC，对乳房炎症的诊断具有重要价值。
BackgroundMilk acute phase proteins (APP) have been identified and show promise as biomarkers of mastitis. However analysis of their profile in dairy cows from a production herd is necessary in order to confirm their benefits in mastitis diagnosis. The profiles of milk haptoglobin (Hp), mammary associated serum amyloid A3 (M-SAA3) and C-reactive protein (CRP) were determined in 54 composite milk (milk from all functional quarters of a cow’s udder collected in a common receptacle) samples (CMS) from a commercial dairy farm. Milk Hp was also determined in individual quarter milk (milk from a single udder quarter) samples (QMS) (n = 149) of the cows.An ELISA was developed and validated for the determination of milk Hp while commercial kits were used for M-SAA3 and CRP assay respectively. Composite milk APP results were compared with cow factors including parity, stage of lactation, percentage protein and fat as well as somatic cell counts (SCC).ResultsComposite milk Hp ranged from <0.4–55 μg/ml with a median of 3.5 μg/ml; composite milk M-SAA3 ranged from <0.6–50 μg/ml and had a median of 1.2 μg/ml, while CRP ranged from <1.80–173 ng/ml and had a median of 24.6 ng/ml. Significant correlations were found between composite SCC and Hp (P-value <0.009) as well as parity and Hp (P < 0.009), but not between M-SAA3 and SCC, M-SAA3 and Hp, M-SAA3 and CRP or M-SAA3 and parity. Milk CRP was correlated with % fat (P = 0.002) and % protein (P = 0.001) of the milk samples. The lack of correlation of SCC with the M-SAA3 and CRP could result from these APP being more sensitive to intra-mammary infection than SCC. Quarter milk Hp had a range of <0.4–420 μg/ml with a median value of 3.6 μg/ml, with 92 % of samples below 20 μg/ml.ConclusionBaseline values of Hp, M-SAA3 and CRP were established in composite milk from cows with normal SCC on the dairy farm. Parity was recognized as a possible confounding factor when diagnosing mastitis using Hp. The value of the APP, Hp, M-SAA3 and CRP as substitutes or to complement SCC in indicating udder inflammation, was demonstrated.
牛（Bos taurus）卵巢和输卵管组织和体液中触珠蛋白样蛋白的鉴定和免疫组织化学定位。
DOI：
10.1530/rep.0.1250837
发表时间：
2003
期刊：
Reproduction (Cambridge, England)
影响因子：
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作者：
Lavery,K;Way,A;Killian,G
通讯作者：
Killian,G